Abstract：
Cell-based assays, conducted on monolayer (2D) cultured cells, are an unquestionably valuable tool for biomedical research. However, three-dimensional (3D) cell culture models have gained relevance over the last few years due to the advantages of better mimicking the microenvironment and tissue microarchitecture in vivo. Recent magnetic-based 3D (m3D) cell culture systems can be used for this purpose. These systems are based on exposing magnetized cells to magnetic fields by levitation, bioprinting, or ring formation to promote cell aggregation into 3D structures. However, the successful development of these structures is dependent on several methodological characteristics and can be applied to mimic different human tissues. Thus, a systematic review was performed using Medline (via Pubmed), Scopus, and Web of Science (until February 2022) databases to aggregate studies using m3D culture in which human tissues were mimicked. The search generated 3784 records, of which 25 met the inclusion criteria. The usability of these m3D systems for the development of homotypic or heterotypic spheroids with or without scaffolds was explored in these studies. We also explore methodological differences specifically related to the magnetic method. Generally, the development of m3D cultures has been increasing, with bioprinting and levitation systems being the most used to generate homotypic or heterotypic cultures, mainly to mimic the physiology of human tissues, but also to perform therapeutic screening. This systematic review showed that there are areas of research where the application of this method remains barely explored, such as cancer research.
摘要：
基于细胞的检测，在单层（2D）培养的细胞上进行，无疑是生物医学研究的宝贵工具。然而,由于在体内更好地模拟微环境和组织微结构的优点,三维(3D)细胞培养模型在过去几年中获得了相关性。最近的基于磁性的3D(m3D)细胞培养系统可用于此目的。这些系统基于通过悬浮将磁化细胞暴露在磁场中，生物打印,或环形成以促进细胞聚集成3D结构。然而,这些结构的成功发展取决于几个方法学特征，可以应用于模拟不同的人体组织。因此，使用Medline（通过Pubmed）进行系统评价，Scopus,和WebofScience（至2022年2月）数据库，以使用模仿人体组织的m3D培养物进行汇总研究。搜索生成了3784条记录，其中25人符合纳入标准。在这些研究中探索了这些m3D系统用于开发具有或不具有支架的同型或异型球体的可用性。我们还探讨了与磁性方法特别相关的方法差异。一般来说,m3D文化的发展一直在增加，生物打印和悬浮系统最常用于产生同型或异型培养物，主要是模仿人体组织的生理学，还要进行治疗性筛查。这篇系统的综述表明，在某些研究领域，这种方法的应用还很少被探索，比如癌症研究。
