Abstract：
Herein, we introduced a novel individual sperm freezing device named SpermCD, which consists of a right angular cryopiece (RA-Cryopiece, or \"C\") and a grooved petri dish (\"D\"). SpermCD allows embryologists to transfer sperm and perform ICSI on the same focal plane. Thirty-five patients underwent single sperm cryopreservation using SpermCD, including four patients with non-obstructive azoospermia (NOA), 14 patients with virtual azoospermia and 17 patients with cryptozoospermia. One hundred and twenty-five cryopreserved spermatozoa from nine patients were thawed on the day of the oocyte retrieval and 121 spermatozoa were found, with a sperm recovery rate of 97.1 ± 4.6%. Sixty-five MII oocytes from their spouse were injected with thawed sperm. Normal fertilization and high-quality embryo rates were 68.0% ± 33.2% and 24.4% ± 22.2%. Nineteen transplantable embryos were formed after fertilization with frozen sperm, eight of which were transplanted in five couples, resulting in four successful deliveries. SpermCD is a simple and practical individual sperm freezing device.
摘要：
在这里，我们介绍了一种名为SpermCD的新型个体精子冷冻装置，它由一个直角的cryopiece(RA-Cryopiece，或“C”）和带凹槽的培养皿（“D”）。SpermCD允许胚胎学家转移精子并在同一焦平面上进行ICSI。35例患者使用SpermCD进行了单个精子冷冻保存，包括4例非梗阻性无精子症（NOA）患者，虚拟无精子症14例，隐精子症17例。取卵当天解冻了9名患者的125个冷冻保存的精子，发现了121个精子，精子回收率为97.1±4.6%。向配偶的65个MII卵母细胞注射解冻的精子。正常受精和优质胚胎率分别为68.0%±33.2%和24.4%±22.2%。用冷冻精子受精后形成了19个可移植的胚胎，其中八对是五对夫妇移植的，导致四次成功交付。SpermCD是一种简单实用的个体精子冷冻装置。
