Abstract：
Analyses of fungal genomes of escalate from biological and evolutionary investigations. The biochemical analyses of putative enzymes will inevitably lag behind and only a selection will be characterized. Plant-pathogenic fungi secrete manganese-lipoxygenases (MnLOX), which oxidize unsaturated fatty acids to hydroperoxides to support infection. Six MnLOX have been characterized so far including the 3D structures of these enzymes of the Rice blast and the Take-all fungi. The goal was to use this information to evaluate MnLOX-related gene transcripts to find informative specimens for further studies. Phylogenetic analysis, determinants of catalytic activities, and the C-terminal amino acid sequences divided 54 transcripts into three major subfamilies. The six MnLOX belonged to the same \"prototype\" subfamily with conserved residues in catalytic determinants and C-terminal sequences. The second subfamily retained the secretion mechanism, presumably necessary for uptake of Mn2+, but differed in catalytic determinants and by cysteine replacement of an invariant Leu residue for positioning (\"clamping\") of fatty acids. The third subfamily contrasted with alanine in the Gly/Ala switch for regiospecific oxidation and a minority contained unprecedented C-terminal sequences or lacked secretion signals. With these exceptions, biochemical analyses of transcripts of the three subfamilies appear to have reasonable prospects to find active enzymes.
摘要：
从生物学和进化研究中升级的真菌基因组分析。推定的酶的生化分析将不可避免地滞后，并且仅对选择进行表征。植物病原真菌分泌锰-脂氧合酶（MnLOX），将不饱和脂肪酸氧化为氢过氧化物以支持感染。到目前为止，已经对六个MnLOX进行了表征，包括稻瘟病和Take-all真菌的这些酶的3D结构。目的是使用这些信息来评估MnLOX相关的基因转录本，以找到用于进一步研究的信息样本。系统发育分析,催化活性的决定因素，和C端氨基酸序列将54个转录本分为三个主要亚家族。六个MnLOX属于相同的“原型”亚家族，在催化决定簇和C末端序列中具有保守的残基。第二个亚家族保留了分泌机制，推测是吸收Mn2+所必需的，但在催化决定簇和半胱氨酸置换不变的Leu残基以定位（“钳制”）脂肪酸方面有所不同。第三个亚家族与区域特异性氧化的Gly/Ala开关中的丙氨酸形成对比，少数包含前所未有的C末端序列或缺乏分泌信号。除了这些例外,三个亚家族转录本的生化分析似乎具有找到活性酶的合理前景。
