PDF(Pubmed)
Abstract：
Tenuazonic acid (TA) is a highly toxic mycotoxin mainly generated by the fungi of Alternaria genus and widely contaminates agricultural by-products. Given the threat of TA to food-security, it is very important to develop rapid and sensitive detection methods for TA monitoring. In this study, gold nano-particles (AuNP) with average diameter near 17.25 nm were prepared, and the developed AuNP-based strip has an assay time of 15 min with visual limit of detection (LOD) of 12.5 ng/ml and threshold of 100 ng/ml. To further improve sensitivity, multi-branched gold nano-flowers (AuNF) with average diameter near 50 nm were prepared and characterized by UV-VIS and TEM, and the established AuNF-based strip has visual LOD of 0.78 ng/ml and threshold of 50 ng/ml within 15 min. Both assays were applied to determine TA in apple juice and tomato ketchup, and the results were consistent with that of UHPLC-MS/MS. Thus, these assays could be applied for rapid determination of trace TA in real samples.
摘要：
Tenuazonicacid（TA）是一种剧毒的霉菌毒素，主要由链格孢属真菌产生，广泛污染农业副产品。鉴于TA对粮食安全的威胁，建立快速、灵敏的TA检测方法对TA监测具有重要意义。在这项研究中,制备了平均直径接近17.25nm的金纳米粒子(AuNP),并且开发的基于AuNP的条带具有15分钟的测定时间,视觉检测限(LOD)为12.5ng/ml,阈值为100ng/ml。为了进一步提高灵敏度，制备了平均直径在50nm附近的多分支金纳米花（AuNF），并通过UV-VIS和TEM进行了表征，并且建立的基于AuNF的条带在15分钟内具有0.78ng/ml的视觉LOD和50ng/ml的阈值。两种测定法均用于测定苹果汁和番茄酱中的TA，与UHPLC-MS/MS的结果一致。因此，这些检测方法可用于实际样品中痕量TA的快速测定。
