PDF(Pubmed)
Abstract：
Children with autism spectrum disorder (ASD) experience gastrointestinal (GI) issues more frequently and severely than children who are typically developing (TD). The connections between gastrointestinal problems, microbiota, and short-chain fatty acids (SCFAs) in ASD are still being debated. We enrolled 90 children, 45 of whom were diagnosed with ASD, and examined the impact of GI disorders on ASD. The six-item GI Severity Index questionnaire was used to evaluate gastrointestinal symptoms, while the Social Responsiveness Scale was used to evaluate autism symptoms. Further, the Children\'s Sleep Habits Questionnaire and the Children\'s Eating Behavior Questionnaire are used to assess sleep and eating disorders in children. We assessed fecal microbiota by 16S rRNA gene sequencing, and SCFA concentrations by gas chromatography/mass spectrometry. The results revealed that children with ASD exhibited a high rate of gastrointestinal issues (78%), as well as higher rates of social impairment and poor sleeping habits, compared to TD children. However, GI disturbances have a minor impact on autism. In addition, the levels of propionic acid, butyric acid, and valeric acid were significantly higher in the ASD group. Besides, the ASD, TD, and GI subgroups possessed distinct microbiome profiles. These findings suggest that gastrointestinal disturbances have no discernible effect on the core symptoms of autism. Although autism may not cause an increase in GI symptoms directly, alterations in metabolites, such as SCFAs, may cause GI symptoms.
摘要：
患有自闭症谱系障碍（ASD）的儿童比通常发育（TD）的儿童更频繁和严重地经历胃肠道（GI）问题。胃肠道问题之间的联系，微生物群,ASD中的短链脂肪酸（SCFA）仍在争论中。我们招收了90个孩子,其中45人被诊断患有ASD，并检查了胃肠道疾病对ASD的影响。六项GI严重程度指数问卷用于评估胃肠道症状，而社会反应量表用于评估自闭症症状。Further,儿童睡眠习惯问卷和儿童饮食行为问卷用于评估儿童的睡眠和饮食障碍。我们通过16SrRNA基因测序评估粪便微生物群，和SCFA浓度通过气相色谱/质谱分析。结果显示，患有ASD的儿童表现出很高的胃肠道问题(78%)，以及较高的社会损害率和不良的睡眠习惯，与TD儿童相比。然而,胃肠道紊乱对自闭症有较小的影响。此外,丙酸的水平，丁酸,和戊酸在ASD组中显著增高。此外,ASD,TD,和GI亚组具有不同的微生物组谱。这些发现表明，胃肠道紊乱对自闭症的核心症状没有明显的影响。虽然自闭症可能不会直接导致胃肠道症状的增加，代谢物的改变，例如SCFA，可能导致胃肠道症状。
