Abstract：
Suspension arrays are a critical components of next generation multiplexed detection technologies. Current fluorescence suspension arrays are limited by a multiplexed coding ceiling and difficulties with ultrasensitive detection. Raman mode is a promising substitute, but the complex spectral peak distributions and extremely weak intrinsic signal intensity severely diminish Raman signal performance in suspension arrays. To address these limitations, we constructed a Raman suspension array system using plasmonic microbeads as barcode substrates and Au nanoflowers as reporter carriers. The well-designed shell morphology and plasmonic microbead composition enabled significant surface enhancement Raman scattering (SERS) such that we were able to adjust silent region Raman-coding intensity levels. Due to synergistic SERS effects from the plasmonic shell and the multi-branched Au nanoflower nanostructure, the reporting signal was greatly improved, enabling ultrasensitive detection of 5-plexed lung cancer markers. Detection in patient serum samples demonstrated good consistency with the standard electrochemiluminescence method. Thus, this silent region SERS barcode-based suspension array is a developmental advance for modern multiplexed biodetection, potentially providing a powerful early disease screening and diagnosis tool.
摘要：
悬浮阵列是下一代多路检测技术的关键组成部分。当前的荧光悬浮液阵列受到多重编码上限和超灵敏检测困难的限制。拉曼模式是一个很有前途的替代品，但是复杂的光谱峰分布和极弱的固有信号强度严重降低了悬浮阵列中的拉曼信号性能。为了解决这些限制，我们使用等离子体微珠作为条形码基底和Au纳米花作为报告载体构建了拉曼悬浮阵列系统。精心设计的壳形态和等离子体微珠组合物实现了显着的表面增强拉曼散射（SERS），因此我们能够调整无声区域拉曼编码强度水平。由于等离子体壳和多分支Au纳米壳纳米结构的协同SERS效应，报告信号大大改善，能够超灵敏地检测5-plex肺癌标志物。在患者血清样品中的检测显示出与标准电化学发光方法良好的一致性。因此，这种基于沉默区域SERS条形码的悬浮阵列是现代多重生物检测的发展进步，可能提供一个强大的早期疾病筛查和诊断工具。
