PDF(Pubmed)
Abstract：
BACKGROUND: Rhizosphere fungi and endophytic fungi play key roles in plant growth and development; however, their role in the growth of Epimedium koreanum Nakai at different stages remains unclear. Here, we used the Illumina MiSeq system, a high-throughput sequencing technology, to study the endophytic fungi and rhizosphere microbiome of Korean Epimedium.
RESULTS: Epimedium koreanum Nakai rhizosphere soil and leaves had highly diverse fungal communities during the growth process. The relative abundance of soil fungi in the rhizosphere stage was higher than that of leaf endophytic fungi in the early growth stage, but the overall abundance was basically equal. Sebacina is a significantly divergent fungal genera, and Sebacina sp. are present among leaf fungi species in the rhizosphere soil of Epimedium koreanum Nakai. Sebacina sp. can move to each other in rhizosphere soil fungi and leaf endophytes. VIF (variance inflation factor) analysis showed that soluble salt, whole nitrogen, alkaline lysis nitrogen, whole phosphorus, total potassium, and fast-acting potassium are useful environmental factors for rhizosphere soil and leaf endophytic fungi: potassium, total nitrogen, whole phosphorus, and three environmental factors were significantly and positively associated with the relative abundance of Sebacina sp.
CONCLUSIONS: (1) This study is the first to clarify the species diversity of fungi in Epimedium koreanum Nakai leaf and rhizosphere soil. (2) Different fungal communities of rhizosphere soil fungi and leaf endophytic fungi at different growth stages of Epimedium koreanum Nakai were examined. (3) Sebacina sp. can move to each other between rhizosphere soil fungi and leaf endophytic fungi. (4) Nitrogen, phosphorus and potassium elements in the environment have a significant positive effect on the relative abundance of Sebacina sp.
RESULTS: Epimedium koreanum Nakai rhizosphere soil and leaves had highly diverse fungal communities during the growth process. The relative abundance of soil fungi in the rhizosphere stage was higher than that of leaf endophytic fungi in the early growth stage, but the overall abundance was basically equal. Sebacina is a significantly divergent fungal genera, and Sebacina sp. are present among leaf fungi species in the rhizosphere soil of Epimedium koreanum Nakai. Sebacina sp. can move to each other in rhizosphere soil fungi and leaf endophytes. VIF (variance inflation factor) analysis showed that soluble salt, whole nitrogen, alkaline lysis nitrogen, whole phosphorus, total potassium, and fast-acting potassium are useful environmental factors for rhizosphere soil and leaf endophytic fungi: potassium, total nitrogen, whole phosphorus, and three environmental factors were significantly and positively associated with the relative abundance of Sebacina sp.
CONCLUSIONS: (1) This study is the first to clarify the species diversity of fungi in Epimedium koreanum Nakai leaf and rhizosphere soil. (2) Different fungal communities of rhizosphere soil fungi and leaf endophytic fungi at different growth stages of Epimedium koreanum Nakai were examined. (3) Sebacina sp. can move to each other between rhizosphere soil fungi and leaf endophytic fungi. (4) Nitrogen, phosphorus and potassium elements in the environment have a significant positive effect on the relative abundance of Sebacina sp.
摘要：
背景：根际真菌和内生真菌在植物生长和发育中起着关键作用；然而，它们在朝鲜淫羊藿不同阶段的生长中的作用尚不清楚。这里,我们使用了IlluminaMiSeq系统,高通量测序技术，研究韩国淫羊藿内生真菌和根际微生物组。
结果：朝鲜淫羊藿根际土壤和叶片在生长过程中具有高度多样性的真菌群落。根际期土壤真菌的相对丰度高于生长早期的叶片内生真菌,但总体丰度基本相等。Sebacina是一个明显不同的真菌属，和Sebacinasp.存在于朝鲜淫羊藿根际土壤中的叶真菌物种中。Sebacinasp.根际土壤真菌和叶片内生菌可以相互移动。VIF(方差膨胀因子)分析表明，可溶性盐、全氮,碱解氮，全磷,总钾，和速效钾是根际土壤和叶片内生真菌的有用环境因素：钾，总氮,全磷,三个环境因素与Sebacinasp的相对丰度呈显着正相关。
结论：（1）本研究首次阐明了朝鲜淫羊藿叶片和根际土壤中真菌的物种多样性。(2)研究了朝鲜淫羊藿不同生长阶段根际土壤真菌和叶片内生真菌的不同真菌群落。(3)Sebacinasp.可以在根际土壤真菌和叶片内生真菌之间相互移动。(4)氮气,环境中的磷和钾元素对Sebacinasp的相对丰度有显着的正向影响。
结果：朝鲜淫羊藿根际土壤和叶片在生长过程中具有高度多样性的真菌群落。根际期土壤真菌的相对丰度高于生长早期的叶片内生真菌,但总体丰度基本相等。Sebacina是一个明显不同的真菌属，和Sebacinasp.存在于朝鲜淫羊藿根际土壤中的叶真菌物种中。Sebacinasp.根际土壤真菌和叶片内生菌可以相互移动。VIF(方差膨胀因子)分析表明，可溶性盐、全氮,碱解氮，全磷,总钾，和速效钾是根际土壤和叶片内生真菌的有用环境因素：钾，总氮,全磷,三个环境因素与Sebacinasp的相对丰度呈显着正相关。
结论：（1）本研究首次阐明了朝鲜淫羊藿叶片和根际土壤中真菌的物种多样性。(2)研究了朝鲜淫羊藿不同生长阶段根际土壤真菌和叶片内生真菌的不同真菌群落。(3)Sebacinasp.可以在根际土壤真菌和叶片内生真菌之间相互移动。(4)氮气,环境中的磷和钾元素对Sebacinasp的相对丰度有显着的正向影响。
