PDF(Pubmed)
Abstract：
Cardiac arrhythmias are a major cause of morbidity and mortality worldwide. Although recent advances in cell-based models, including human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM), are contributing to our understanding of electrophysiology and arrhythmia mechanisms, preclinical animal studies of cardiovascular disease remain a mainstay. Over the past several decades, animal models of cardiovascular disease have advanced our understanding of pathological remodeling, arrhythmia mechanisms, and drug effects and have led to major improvements in pacing and defibrillation therapies. There exist a variety of methodological approaches for the assessment of cardiac electrophysiology and a plethora of parameters may be assessed with each approach. This guidelines article will provide an overview of the strengths and limitations of several common techniques used to assess electrophysiology and arrhythmia mechanisms at the whole animal, whole heart, and tissue level with a focus on small animal models. We also define key electrophysiological parameters that should be assessed, along with their physiological underpinnings, and the best methods with which to assess these parameters.
摘要：
心律失常是世界范围内发病率和死亡率的主要原因。尽管基于细胞的模型最近取得了进展，包括人诱导的多能干细胞衍生的心肌细胞（iPSC-CM），有助于我们对电生理学和心律失常机制的理解，心血管疾病的临床前动物研究仍然是一个支柱。在过去的几十年里，心血管疾病的动物模型提高了我们对病理性重塑的认识，心律失常机制,和药物作用，并导致了起搏和除颤治疗的重大改进。存在多种用于评估心脏电生理的方法学方法,并且可以用每种方法评估过多的参数。本指南文章将概述用于评估整个动物的电生理和心律失常机制的几种常用技术的优势和局限性。整个心脏,和组织水平，重点是小动物模型。我们还定义了应该评估的关键电生理参数，以及它们的生理基础，以及评估这些参数的最佳方法。
