Mesh : Humans Child Streptococcus mutans / metabolism Cross-Sectional Studies Adhesins, Bacterial / metabolism Dental Caries Collagen / metabolism DNA / metabolism Saliva / metabolism

来  源:   DOI:10.1038/s41598-022-22378-8

Abstract:
Streptococcus mutans is a major etiological agent for dental caries. We previously demonstrated that S. mutans strains expressing collagen-binding proteins (CBPs) were related to the pathogenesis of systemic diseases. However, their acquisition and colonization remain unknown. Here, we investigated the detection rates of CBP-positive S. mutans strains in children and their guardians to clarify the background for the acquisition and colonization in children. Saliva samples were collected from children and their mothers, and detection of S. mutans and collagen-binding genes (cnm, cbm) was performed by PCR after DNA extraction. The oral status of each child was examined, and their mothers were asked to complete a questionnaire. The isolation rate of Cnm-positive S. mutans was significantly higher in mothers than in children. Notably, the possession rates of CBP-positive strains in children were significantly higher in children whose mothers had CBP-positive strains than in children whose mothers did not have these strains. Furthermore, children with CBP-positive strains had a significantly shorter breastfeeding period than children without these strains. The present results suggest that nutritional feeding habits in infancy are one of the factors involved in the acquisition and colonization of CBP-positive S. mutans strains.
摘要:
变形链球菌是龋齿的主要病因。我们先前证明了表达胶原蛋白结合蛋白(CBP)的变形链球菌菌株与全身性疾病的发病机理有关。然而,他们的获取和殖民仍然未知。这里,我们调查了CBP阳性变异链球菌在儿童及其监护人中的检出率,以阐明儿童获得和定植的背景.唾液样本是从儿童和他们的母亲那里收集的,并检测变形链球菌和胶原蛋白结合基因(cnm,cbm)在DNA提取后通过PCR进行。检查了每个孩子的口腔状况,他们的母亲被要求填写一份问卷。母亲Cnm阳性变形链球菌的分离率明显高于儿童。值得注意的是,母亲患有CBP阳性菌株的儿童中CBP阳性菌株的持有率显著高于母亲没有这些菌株的儿童.此外,有CBP阳性菌株的儿童的母乳喂养期明显短于没有这些菌株的儿童.目前的结果表明,婴儿期的营养摄食习惯是参与CBP阳性变形链球菌菌株获得和定植的因素之一。
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