Abstract：
The low production of natural products (NPs) is still the critical restrictive factor in exploiting their potential large-scale applications and a barrier to isolating and identifying other meaningful products. Given that the stimulation of cell wall integrity (CWI) has become a novel strategy to modulate the production of microbial natural products, herein, exogenous β-glucanase treatment was developed as an external cell wall β-glucan stress to stimulate the fungal CWI, and then to improve the production of fungal NPs. It was found that the production of fungal NPs cercosporin and sophorolipids, biosynthesized by Cercospora sp. and Starmerella bombicola, respectively, was significantly improved by the treatment of β-glucanase under a controllable dose. Moreover, it demonstrated that β-glucanase had an ability to stimulate fungal CWI through slight fungal superficial damage, thus facilitating the secretion of NPs. We expected that this easy-operating method to stimulate fungal CWI could be feasible to improve more fungal NPs production. KEY POINTS: • Exogenous β-glucanase stimulated the fungal cell wall integrity • Changing fungal cell walls modulated natural product production • β-glucanase with potential universal effects on more fungal natural products.
摘要：
天然产物（NPs）的低产量仍然是开发其潜在大规模应用的关键限制因素，也是分离和识别其他有意义产品的障碍。鉴于刺激细胞壁完整性（CWI）已成为调节微生物天然产物生产的新策略，在这里，外源β-葡聚糖酶处理被开发为外部细胞壁β-葡聚糖应激来刺激真菌CWI，然后提高真菌NPs的产量。发现真菌NPscercosporin和槐糖脂的产生，Cercosporasp。和Starmerellabombicola,分别,在可控剂量下通过β-葡聚糖酶的处理显着改善。此外，它表明β-葡聚糖酶具有通过轻微的真菌表面损伤刺激真菌CWI的能力，从而促进NPs的分泌。我们预计这种简单的方法来刺激真菌CWI可能是可行的，以提高更多的真菌NPs的生产。关键点:•外源性β-葡聚糖酶刺激真菌细胞壁完整性•改变真菌细胞壁调节天然产物生产•β-葡聚糖酶对更多真菌天然产物具有潜在的普遍影响。
