Abstract：
This study aimed to evaluate the antibacterial effect of three final irrigation protocols and to compare their ability to remove the smear layer and debris from the root canal. Methods: Sixty-three single-rooted human teeth were inoculated with Enterococcus faecalis for 14 days. The teeth were divided into a positive control group (N = 3) and three treatment groups (N = 20) as follows: final irrigation with saline solution (control group), irrigation with 5.25% NaOCl ultrasonically activated with EndoUltra (EU), standard needle irrigation with Qmix 2in1 (Qx) and irrigation with 5.25% NaOCl activated using SiroLaser Blue (SB). The bacterial load was evaluated by analyzing the colony-forming units (CFU/mL). Selected specimens were split longitudinally and examined using scanning electron microscopy in order to determine the presence of a smear layer and debris. Statistical analyses were performed using one-way ANOVA and the Kruskal−Wallis rank-sum test. Results: Activation of NaOCl with EndoUltra or SiroLaser Blue was superior at reducing intracanal bacteria relative to standard needle irrigation with Qmix 2in1 solution (p < 0.05). Even though SiroLaser Blue showed the best results for removing the smear layer and debris, no significant differences were detected between the groups (p > 0.05). Conclusions: Final irrigation with 5.25% NaOCl ultrasonically activated using EndoUltra or SiroLaser Blue demonstrated a higher efficacy in bacterial reduction than standard needle irrigation with Qmix 2in1.
摘要：
这项研究旨在评估三种最终灌溉方案的抗菌效果，并比较它们从根管中去除涂抹层和碎屑的能力。
方法：63颗单根人牙接种粪肠球菌14天。将牙齿分为阳性对照组（N=3）和三个治疗组（N=20），如下：用盐溶液进行最终冲洗（对照组），用EndoUltra（EU）超声激活的5.25％NaOCl灌溉，用Qmix2in1（Qx）进行标准针头冲洗，并用SiroLaserBlue（SB）激活的5.25％NaOCl进行冲洗。通过分析菌落形成单位(CFU/mL)评估细菌载量。将选定的标本纵向分开并使用扫描电子显微镜检查以确定涂抹层和碎片的存在。使用单向ANOVA和Kruskal-Wallis秩和检验进行统计分析。
结果：使用EndoUltra或SiroLaserBlue对NaOCl的活化在减少肛门内细菌方面优于使用Qmix2in1溶液的标准针冲洗（p结论：使用EndoUltra或SiroLaserBlue超声激活的5.25％NaOCl最终冲洗比使用Qmix2in1的标准针头冲洗更有效。
方法：63颗单根人牙接种粪肠球菌14天。将牙齿分为阳性对照组（N=3）和三个治疗组（N=20），如下：用盐溶液进行最终冲洗（对照组），用EndoUltra（EU）超声激活的5.25％NaOCl灌溉，用Qmix2in1（Qx）进行标准针头冲洗，并用SiroLaserBlue（SB）激活的5.25％NaOCl进行冲洗。通过分析菌落形成单位(CFU/mL)评估细菌载量。将选定的标本纵向分开并使用扫描电子显微镜检查以确定涂抹层和碎片的存在。使用单向ANOVA和Kruskal-Wallis秩和检验进行统计分析。
结果：使用EndoUltra或SiroLaserBlue对NaOCl的活化在减少肛门内细菌方面优于使用Qmix2in1溶液的标准针冲洗（p
