PDF(Pubmed)
Abstract：
Peptidoglycan recognition proteins (PGRPs) are a family of pattern recognition receptors (PRRs) involved in host antibacterial responses, and their functions have been characterized in most invertebrate and vertebrate animals. However, little information is available regarding the potential function of PGRPs in the giant triton snail Charonia tritonis. In this study, a short-type PGRP gene (termed Ct-PGRP-S1) was identified in C. tritonis. Ct-PGRP-S1 was predicted to contain several structural features known in PGRPs, including a typical PGRP domain (Amidase_2) and Src homology-3 (SH3) domain. The Ct-PGRP-S1 gene was constitutively expressed in all tissues examined except in proboscis, with the highest expression level observed in the liver. As a typical PRR, Ct-PGRP-S1 has an ability to degrade peptidoglycan (PGN) and was proven to have non-Zn2+-dependent amidase activity and antibacterial activity against Vibrioalginolyticus and Staphylococcus aureus. It is the first report to reveal the peptidoglycan recognition protein in C. tritonis, and these results suggest that peptidoglycan recognition protein Ct-PGRP-S1 is an important effector of C. tritonis that modulates bacterial infection resistance of V. alginolyticus and S. aureus, and this study may provide crucial basic data for the understanding of an innate immunity system of C. tritonis.
摘要：
肽聚糖识别蛋白（PGRP）是参与宿主抗菌反应的模式识别受体（PRR）家族，它们的功能在大多数无脊椎动物和脊椎动物中都有特点。然而,关于巨大的Triton蜗牛Charoniatritonis中PGRP的潜在功能的信息很少。在这项研究中,短型PGRP基因(称为Ct-PGRP-S1)在三棒状杆菌中被鉴定。预测Ct-PGRP-S1包含PGRP中已知的几种结构特征，包括典型的PGRP结构域(酰胺酶_2)和Src同源-3(SH3)结构域。Ct-PGRP-S1基因在所有检查的组织中组成型表达，除了在长鼻中，在肝脏中观察到最高的表达水平。作为典型的PRR，Ct-PGRP-S1具有降解肽聚糖（PGN）的能力，并被证明具有非Zn2依赖性酰胺酶活性和对溶藻弧菌和金黄色葡萄球菌的抗菌活性。这是第一份揭示三维甲酸杆菌中肽聚糖识别蛋白的报告，这些结果表明，肽聚糖识别蛋白Ct-PGRP-S1是三嗜血杆菌的重要效应因子，可调节溶藻弧菌和金黄色葡萄球菌的细菌感染抗性，这项研究可能为了解三嗜血杆菌的先天免疫系统提供重要的基础数据。
