Abstract：
Arachidonic acid is an essential ω-6 polyunsaturated fatty acid, which plays a significant role in cardiovascular health and neurological development, leading to its wide use in the food and pharmaceutical industries. Traditionally, ARA is obtained from deep-sea fish oil. However, this source is limited by season and is depleting the already threatened global fish stocks. With the rapid development of synthetic biology in recent years, oleaginous fungi have gradually attracted increasing attention as promising microbial sources for large-scale ARA production. Numerous advanced technologies including metabolic engineering, dynamic regulation of fermentation conditions, and multiomics analysis were successfully adapted to increase ARA synthesis. This review summarizes recent advances in the bioengineering of oleaginous fungi for ARA production. Finally, perspectives for future engineering approaches are proposed to further improve the titer yield and productivity of ARA.
摘要：
花生四烯酸是一种必需的ω-6多不饱和脂肪酸，在心血管健康和神经发育中起着重要作用，导致其在食品和制药行业的广泛应用。传统上,ARA从深海鱼油获得。然而,这种来源受季节限制，正在耗尽已经受到威胁的全球鱼类资源。近年来,随着合成生物学的迅速发展,含油真菌作为用于大规模ARA生产的有希望的微生物来源已逐渐引起越来越多的关注。许多先进的技术，包括代谢工程，发酵条件的动态调节，和多组学分析成功地适应增加ARA合成。这篇综述总结了用于ARA生产的产油真菌生物工程的最新进展。最后,提出了未来工程方法的观点，以进一步提高ARA的滴度产量和生产率。
