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Abstract：
The present study was designed to evaluate the association of polymorphisms in bovine trafficking protein particle complex subunit 9 (TRAPPC9) and cluster of differentiation 4 (CD4) genes with milk production and mastitis resistance phenotypic traits in a different cattle population. Three single nucleotide polymorphisms (SNPs) (SNP1 Position: Chr14:2484891, SNP2 (rs110017379), SNP3 Position: Chr14:2525852) in bovine TRAPPC9 and one SNP (Position: Chr5:104010752) in CD4 were screened through Chinese Cow\'s SNPs Chip-I (CCSC-I) and genotyped in a population of 312 Chinese Holsteins (156: Mastitis, 156: Healthy). The results were analyzed using the general linear model in SAS 9.4. Our analysis revealed that milk protein percentage, somatic cell count (SCC), somatic cell score (SCS), serum cytokines interleukin 6 (IL-6) and interferon-gamma (IFN-γ) were significantly (P < 0.05) associated with at least one or more identified SNPs of TRAPPC9 and CD4 genes. Furthermore, the expression status of SNPs in CD4 and TRAPPC9 genes were verified through RT-qPCR. The expression analysis showed that genotypes GG in SNP3 of TRAPPC9 and TT genotype in SNP4 of CD4 showed higher expression level compared to other genotypes. The GG genotype in SNP2 and TT genotype in SNP3 of TRAPPC9 were associated with higher bovine milk SCC and lower IL6. Altogether, our findings suggested that the SNPs of TRAPPC9 and CD4 genes could be useful genetic markers in selection for milk protein improvement and mastitis resistance phenotypic traits in dairy cattle. The CCSC-I used in current study is proposed to be validate in different and large population of dairy cattle not only in China but also in other countries. Moreover, our analyses recommended that besides SCC and SCS, the association of genetic markers could also be considered with the serum cytokines (IL-6, IFN-γ) while selecting genetically mastitis resistance dairy cattle.
摘要：
本研究旨在评估不同牛群中牛运输蛋白颗粒复合物亚基9（TRAPPC9）和分化簇4（CD4）基因的多态性与产奶量和乳腺炎抗性表型性状的关联。三个单核苷酸多态性（SNPs）（SNP1位置：Chr14：2484891，SNP2（rs110017379），通过中国牛SNPs芯片-I（CMSC-I）筛选牛TRAPPC9中的SNP3位置：Chr14：2525852）和CD4中的一个SNP（位置：Chr5：104010752），并在312个中国荷斯坦（156：乳腺炎，156：健康）。使用SAS9.4中的一般线性模型对结果进行分析。我们的分析显示牛奶蛋白质百分比，体细胞计数（SCC），体细胞评分（SCS），血清细胞因子白细胞介素6（IL-6）和干扰素-γ（IFN-γ）与至少一个或多个鉴定的TRAPPC9和CD4基因的SNP显着相关（P<0.05）。此外，通过RT-qPCR验证CD4和TRAPPC9基因SNPs的表达状态。表达分析表明，与其他基因型相比，TRAPPC9的SNP3基因型GG和CD4的SNP4基因型TT表达水平更高。TRAPPC9的SNP2中的GG基因型和SNP3中的TT基因型与较高的牛乳SCC和较低的IL6有关。总之,我们的发现表明，TRAPPC9和CD4基因的SNP可能是选择奶牛乳蛋白改善和乳腺炎抗性表型性状的有用遗传标记。本研究中使用的CCSC-I建议不仅在中国而且在其他国家的不同和大量奶牛中进行验证。此外，我们的分析建议，除了SCC和SCS，在选择遗传性乳腺炎抗性乳牛时，也可以考虑遗传标记与血清细胞因子（IL-6，IFN-γ）的关联。
