关键词: Anaerobes Blood cultures Gene sequencing Lancefieldella parvula MALDI TOF MS

Mesh : Humans Aged RNA, Ribosomal, 16S / genetics Bacteremia / diagnosis drug therapy etiology Actinobacteria / genetics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

来  源:   DOI:10.1016/j.anaerobe.2022.102661

Abstract:
We report an uncommon case of bacteremia due to Lancefieldella parvula isolated in pure culture. We present a 72 year-old-man affected with cancer admitted with abdominal pain due to a parietal rupture of the urinary bladder. After surgical treatment, the patient developed fever and two sets of blood cultures yielded a pure culture of an anaerobic microorganism identified as L. parvula (formerly Atopobium parvulum) by MALDI-TOF MS, and confirmed by 16S rRNA gene sequencing. The isolate was susceptible to all antibiotics and the outcome was finally successful. Bacteremia due to L. parvula is an uncommon disease and, in that case, MALDI-TOF MS was an useful tool for the initial identification.
摘要:
我们报告了一例罕见的菌血症,这是由于在纯培养中分离出的Lancefieldella引起的。我们介绍了一名72岁的患有癌症的男子,该男子因膀胱顶叶破裂而因腹痛而入院。手术治疗后,患者出现发烧,两组血液培养物产生了一种厌氧微生物的纯培养物,该厌氧微生物被MALDI-TOFMS鉴定为细小乳杆菌(以前为细小芽孢杆菌),并通过16SrRNA基因测序证实。该分离物对所有抗生素敏感,结果最终成功。小落叶松菌菌血症是一种罕见的疾病,在这种情况下,MALDI-TOFMS是用于初始鉴定的有用工具。
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