PDF(Pubmed)
Abstract：
Extensive use of abamectin (ABM) as an anthelmintic in veterinary systems adversely affects the health and welfare of animals and humans. Zinc nanoparticles (ZnNPs) have therapeutic benefits and ameliorate the effect of environmental pollutants. In this study, we assessed the ameliorative effect of ZnNPs against the sub-lethal toxicity of ABM in rats. Forty healthy rats were randomly selected into four groups (n = 10); the control received normal saline and test rats were treated orally twice weekly with ABM (1 mg/kg bwt), ZnNPs (10 mg/kg bwt) and ABM + ZnNPs for 28 days. Upon completion of the study period, blood and tissue samples were collected and prepared for hematological, biochemical, pathological, and immunohistochemical analysis. Our results showed that ABM treatment significantly decreased body weight gain (BWG), red blood cells (RBCs), hemoglobin (Hb), hematocrit (HC), and platelet (PLT); while it significantly increased white blood cells (WBCs) and lymphocytes. ABM also significantly decreased antioxidant enzyme activities: superoxide dismuthase (SOD), glutathione peroxidase (GPx), and catalase (CAT) and increased hydrogen peroxide and malondialdehyde levels compared with other groups. ABM significantly raised alanine aminotransferase (ALT), aspartate amino transaminase (AST), and alkaline phosphatase (ALP) levels, which was restored by co-administration of ZnNPs. Moreover, ZnNPs ameliorated ABM-mediated negative histopathological changes in the liver and kidney tissues, exhibiting a significant protective effect. Cyclooxygenase 2 (COX-2) + immuno-expression were reduced after pretreatment with ZnNPs. These findings suggested that co-administration of ZnNPs with ABM mitigated its toxicity by combating oxidative stress and boosting antioxidant capacity, indicating the efficacy of ZnNPs in attenuating ABM toxicity.
摘要：
在兽医系统中广泛使用阿维菌素（ABM）作为驱虫药会对动物和人类的健康和福利产生不利影响。锌纳米颗粒(ZnNPs)具有治疗益处并改善环境污染物的影响。在这项研究中,我们评估了ZnNPs对大鼠ABM亚致死毒性的改善作用。将40只健康大鼠随机分为四组（n=10）；对照组接受生理盐水，试验大鼠每周两次口服ABM（1mg/kgbwt），ZnNP(10mg/kgbwt)和ABM+ZnNP持续28天。研究结束后，收集血液和组织样本并准备用于血液学，生物化学，病态,和免疫组织化学分析。我们的结果表明，ABM治疗显着降低体重增加（BWG），红细胞（RBC），血红蛋白（Hb），血细胞比容（HC），和血小板（PLT）;而显着增加白细胞（WBC）和淋巴细胞。ABM还显着降低了抗氧化酶的活性：超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GPx），与其他组相比，过氧化氢酶（CAT）和过氧化氢和丙二醛水平增加。ABM显著升高丙氨酸转氨酶(ALT),天冬氨酸氨基转氨酶（AST），和碱性磷酸酶（ALP）水平，通过共同施用ZnNPs来恢复。此外，ZnNPs改善了ABM介导的肝和肾组织的阴性组织病理学变化，具有明显的保护作用。用ZnNP预处理后，环氧合酶2（COX-2）免疫表达降低。这些发现表明，ZnNPs与ABM共同给药通过对抗氧化应激和提高抗氧化能力来减轻其毒性，表明ZnNPs在减弱ABM毒性方面的功效。
