PDF(Pubmed)
Abstract：
UNASSIGNED: The study aimed to investigate the genome-wide biological significance of the circulating miRNAs markers found in peripheral whole blood of adult epileptic seizures patients by integrating analysis using bioinformatics approaches.
UNASSIGNED: The Gene Expression Omnibus (GEO) dataset was accessed to retrieve epilepsy-related circulating miRNA profile data (GSE114847) including 89 subjects (n = 40 epileptic and n = 49 healthy control), peripheral whole-blood mRNA expression data (GSE143772) including 64 subjects (n = 32 epileptic and n = 32 healthy control). To eliminate age disparities in epilepsy pathophysiology only adult epileptic patients were selected. Furthermore, GEO2R was used to identify adult-related mRNAs (AD-mRNAs) against epilepsy as potential biomarkers. Moreover, to predict the potential target genes for these mRNAs, we used mirWalk. Finally, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were utilized to investigate the biological activities of AD-mRNAs. Importantly, the protein-protein network of these identified AD-mRNAs was constructed. Eventually, the overlapping AD-mRNAs and AD-miRNAs and their functions were explored to shortlist potential AD-epileptic markers.
UNASSIGNED: The current study resulted in the identification of 79 upregulated and 40 downregulated different expression gene (DEGs) in both applied data. These targets were cross-linked and mapped with each other to acquire common adult epilepsy-related overlapped mRNAs (Mo-mRNAs). It was found that there was a total of 36 overlapping genes. These overlapped AD-mRNAs markers were found to be functionally enriched in cell regulating pathways i.e., positive regulation of type 1 interferon signaling pathway and mitochondrial cytochrome C release pathway, respectively.
UNASSIGNED: This research gives a comprehensive depiction of the mRNAs that may be involved in adult epilepsy patients\' pathophysiological progressions.
UNASSIGNED: The Gene Expression Omnibus (GEO) dataset was accessed to retrieve epilepsy-related circulating miRNA profile data (GSE114847) including 89 subjects (n = 40 epileptic and n = 49 healthy control), peripheral whole-blood mRNA expression data (GSE143772) including 64 subjects (n = 32 epileptic and n = 32 healthy control). To eliminate age disparities in epilepsy pathophysiology only adult epileptic patients were selected. Furthermore, GEO2R was used to identify adult-related mRNAs (AD-mRNAs) against epilepsy as potential biomarkers. Moreover, to predict the potential target genes for these mRNAs, we used mirWalk. Finally, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were utilized to investigate the biological activities of AD-mRNAs. Importantly, the protein-protein network of these identified AD-mRNAs was constructed. Eventually, the overlapping AD-mRNAs and AD-miRNAs and their functions were explored to shortlist potential AD-epileptic markers.
UNASSIGNED: The current study resulted in the identification of 79 upregulated and 40 downregulated different expression gene (DEGs) in both applied data. These targets were cross-linked and mapped with each other to acquire common adult epilepsy-related overlapped mRNAs (Mo-mRNAs). It was found that there was a total of 36 overlapping genes. These overlapped AD-mRNAs markers were found to be functionally enriched in cell regulating pathways i.e., positive regulation of type 1 interferon signaling pathway and mitochondrial cytochrome C release pathway, respectively.
UNASSIGNED: This research gives a comprehensive depiction of the mRNAs that may be involved in adult epilepsy patients\' pathophysiological progressions.
摘要：
UNASSIGNED：该研究旨在通过使用生物信息学方法进行整合分析，研究在成人癫痫发作患者的外周全血中发现的循环miRNA标记的全基因组生物学意义。
UNASSIGNED：访问了基因表达综合（GEO）数据集，以检索与癫痫相关的循环miRNA谱数据（GSE114847），其中包括89名受试者（n=40例癫痫患者和n=49例健康对照），外周全血mRNA表达数据（GSE143772），包括64名受试者（n=32例癫痫患者和n=32例健康对照）。为了消除癫痫病理生理学中的年龄差异，仅选择成年癫痫患者。此外，GEO2R用于鉴定针对癫痫的成人相关mRNA(AD-mRNA)作为潜在的生物标志物。此外，预测这些mRNA的潜在靶基因，我们用的是mirWalk.最后,利用基因本体论（GO）和京都基因和基因组百科全书（KEGG）来研究AD-mRNA的生物学活性。重要的是,构建了这些鉴定的AD-mRNA的蛋白质-蛋白质网络。最终,研究了重叠的AD-mRNAs和AD-miRNAs及其功能,以筛选出潜在的AD-癫痫标志物.
UNASSIGNED:本研究结果在两个应用数据中鉴定出79个上调和40个下调的不同表达基因(DEGs)。这些靶标彼此交联并定位以获得常见的成人癫痫相关重叠的mRNA(Mo-mRNA)。发现共有36个重叠基因。发现这些重叠的AD-mRNA标记在细胞调节途径中功能富集，即正调控1型干扰素信号通路和线粒体细胞色素C释放通路,分别。
未经证实：这项研究全面描述了可能与成人癫痫患者病理生理进展有关的mRNA。
UNASSIGNED：访问了基因表达综合（GEO）数据集，以检索与癫痫相关的循环miRNA谱数据（GSE114847），其中包括89名受试者（n=40例癫痫患者和n=49例健康对照），外周全血mRNA表达数据（GSE143772），包括64名受试者（n=32例癫痫患者和n=32例健康对照）。为了消除癫痫病理生理学中的年龄差异，仅选择成年癫痫患者。此外，GEO2R用于鉴定针对癫痫的成人相关mRNA(AD-mRNA)作为潜在的生物标志物。此外，预测这些mRNA的潜在靶基因，我们用的是mirWalk.最后,利用基因本体论（GO）和京都基因和基因组百科全书（KEGG）来研究AD-mRNA的生物学活性。重要的是,构建了这些鉴定的AD-mRNA的蛋白质-蛋白质网络。最终,研究了重叠的AD-mRNAs和AD-miRNAs及其功能,以筛选出潜在的AD-癫痫标志物.
UNASSIGNED:本研究结果在两个应用数据中鉴定出79个上调和40个下调的不同表达基因(DEGs)。这些靶标彼此交联并定位以获得常见的成人癫痫相关重叠的mRNA(Mo-mRNA)。发现共有36个重叠基因。发现这些重叠的AD-mRNA标记在细胞调节途径中功能富集，即正调控1型干扰素信号通路和线粒体细胞色素C释放通路,分别。
未经证实：这项研究全面描述了可能与成人癫痫患者病理生理进展有关的mRNA。
