Abstract：
The recent development of small, single-amplicon-based benchtop systems for pyrosequencing has opened up a host of novel procedures for applications in forensic science. Pyrosequencing is a sequencing by synthesis technique, based on chemiluminescent inorganic pyrophosphate detection. This review explains the pyrosequencing workflow and illustrates the step-by-step chemistry, followed by a description of the assay design and factors to keep in mind for an exemplary assay. Existing and potential forensic applications are highlighted using this technology. Current applications include identifying species, identifying bodily fluids, and determining smoking status. We also review progress in potential applications for the future, including research on distinguishing monozygotic twins, detecting alcohol and drug abuse, and other phenotypic characteristics such as diet and body mass index. Overall, the versatility of the pyrosequencing technologies renders it a useful tool in forensic genomics.
摘要：
最近的发展小，基于单扩增子的台式焦磷酸测序系统为法医学应用开辟了许多新程序。焦磷酸测序是一种合成测序技术，基于化学发光的无机焦磷酸盐检测。这篇综述解释了焦磷酸测序的工作流程，并说明了逐步的化学，接下来是对示例性测定的测定设计和要记住的因素的描述。使用该技术突出了现有和潜在的取证应用。目前的应用包括识别物种，识别体液，并确定吸烟状况。我们还回顾了未来潜在应用的进展，包括区分单卵双胞胎的研究，检测酒精和药物滥用，和其他表型特征，如饮食和体重指数。总的来说,焦磷酸测序技术的多功能性使其成为法医基因组学的有用工具。
