PDF(Pubmed)
Abstract：
The purpose of the present study was to identify metabolic biomarkers and study the metabolic changes in relation to cataracts and eyeball rupture in human aqueous humor. This case-control study included 3 patients with traumatic ocular rupture treated by surgery as the control group, 10 patients with severe cataracts as the severe cataracts group and 10 patients with mild cataracts as the mild cataracts group. The present study used liquid chromatography-mass spectrometry to analyze the metabolomics of aqueous humor samples. Databases including the Kyoto Encyclopedia of Genes and Genomes and MetaboAnalyst were used to find potential pathways for metabolites. Aqueous humor metabolic spectrum can competently distinguish patients with different degrees of cataracts from the control group. A total of 34 metabolites were identified as potential biomarkers that could distinguish patients with different degrees of cataracts; 36 metabolites were identified as potential biomarkers that could distinguish patients with severe cataracts from the control group; 34 metabolites were identified as potential biomarkers that could distinguish patients with mild cataracts from controls. In pathway analysis, glycerolphospholipid metabolism was highly affected, which meant that these metabolic markers serve an important role in the regulation of this pathway. The present study identified valuable metabolic biomarkers and pathways, which is helpful for an improved understanding of the pathogenesis of cataracts. This discovery has transformation value for the development of new treatment methods for cataracts.
摘要：
本研究的目的是确定代谢生物标志物，并研究与人房水中白内障和眼球破裂有关的代谢变化。本病例对照研究以手术治疗的3例外伤性眼破裂患者为对照组。10例重度白内障患者为重度白内障组，10例轻度白内障患者为轻度白内障组。本研究使用液相色谱-质谱法分析房水样品的代谢组学。包括京都基因和基因组百科全书和MetaboAnalyst在内的数据库用于寻找代谢物的潜在途径。房水代谢谱可以很好地将不同程度的白内障患者与对照组区分开来。共有34种代谢物被鉴定为能够区分不同程度白内障患者的潜在生物标志物；36种代谢物被鉴定为能够区分重度白内障患者和对照组的潜在生物标志物；34种代谢物被鉴定为能够区分轻度白内障患者和对照组的潜在生物标志物。在途径分析中，甘油磷脂代谢受到高度影响，这意味着这些代谢标志物在该途径的调节中起着重要作用。本研究确定了有价值的代谢生物标志物和途径，这有助于更好地理解白内障的发病机制。这一发现对发展白内障新的治疗方法具有转化价值。
