Abstract：
BACKGROUND: To understand the relationship between myocardial contractility and external stimuli, detecting ex vivo myocardial contractility is necessary.
METHODS: We elaborated a method for contractility detection of isolated C57 mouse papillary muscle using Myostation-Intact system under different frequencies, voltages, and calcium concentrations.
RESULTS: The results indicated that the basal contractility of the papillary muscle was 0.27 ± 0.03 mN at 10 V, 500-ms pulse duration, and 1 Hz. From 0.1 to 1.0 Hz, contractility decreased with an increase in frequency (0.45 ± 0.11-0.10 ± 0.02 mN). The voltage-initiated muscle contractility varied from 3 to 6 V, and the contractility gradually increased as the voltage increased from 6 to 10 V (0.14 ± 0.02-0.28 ± 0.03 mN). Moreover, the muscle contractility increased when the calcium concentration was increased from 1.5 to 3 mM (0.45 ± 0.17-1.11 ± 0.05 mN); however, the contractility stopped increasing even when the concentration was increased to 7.5 mM (1.02 ± 0.23 mN).
CONCLUSIONS: Our method guaranteed the survivability of papillary muscle ex vivo and provided instructions for Myostation-Intact users for isolated muscle contractility investigations.
METHODS: We elaborated a method for contractility detection of isolated C57 mouse papillary muscle using Myostation-Intact system under different frequencies, voltages, and calcium concentrations.
RESULTS: The results indicated that the basal contractility of the papillary muscle was 0.27 ± 0.03 mN at 10 V, 500-ms pulse duration, and 1 Hz. From 0.1 to 1.0 Hz, contractility decreased with an increase in frequency (0.45 ± 0.11-0.10 ± 0.02 mN). The voltage-initiated muscle contractility varied from 3 to 6 V, and the contractility gradually increased as the voltage increased from 6 to 10 V (0.14 ± 0.02-0.28 ± 0.03 mN). Moreover, the muscle contractility increased when the calcium concentration was increased from 1.5 to 3 mM (0.45 ± 0.17-1.11 ± 0.05 mN); however, the contractility stopped increasing even when the concentration was increased to 7.5 mM (1.02 ± 0.23 mN).
CONCLUSIONS: Our method guaranteed the survivability of papillary muscle ex vivo and provided instructions for Myostation-Intact users for isolated muscle contractility investigations.
摘要：
背景：为了了解心肌收缩力与外界刺激之间的关系，检测离体心肌收缩力是必要的。
方法：我们阐述了一种使用Myostation-完整系统在不同频率下检测分离的C57小鼠乳头状肌的收缩性的方法，电压,和钙浓度。
结果：结果表明，在10V时，乳头状肌的基础收缩力为0.27±0.03mN，500毫秒脉冲持续时间，和1Hz。从0.1到1.0Hz，收缩力随着频率的增加而降低（0.45±0.11-0.10±0.02mN）。电压引发的肌肉收缩力从3到6V不等，随着电压从6V增加到10V（0.14±0.02-0.28±0.03mN），收缩力逐渐增加。此外，当钙浓度从1.5mM增加到3mM（0.45±0.17-1.11±0.05mN）时，肌肉收缩力增加；但是，即使浓度增加到7.5mM（1.02±0.23mN），收缩力也停止增加。
结论：我们的方法保证了乳头状肌的离体存活性，并为Myostation-intact使用者提供了有关离体肌肉收缩性研究的指导。
方法：我们阐述了一种使用Myostation-完整系统在不同频率下检测分离的C57小鼠乳头状肌的收缩性的方法，电压,和钙浓度。
结果：结果表明，在10V时，乳头状肌的基础收缩力为0.27±0.03mN，500毫秒脉冲持续时间，和1Hz。从0.1到1.0Hz，收缩力随着频率的增加而降低（0.45±0.11-0.10±0.02mN）。电压引发的肌肉收缩力从3到6V不等，随着电压从6V增加到10V（0.14±0.02-0.28±0.03mN），收缩力逐渐增加。此外，当钙浓度从1.5mM增加到3mM（0.45±0.17-1.11±0.05mN）时，肌肉收缩力增加；但是，即使浓度增加到7.5mM（1.02±0.23mN），收缩力也停止增加。
结论：我们的方法保证了乳头状肌的离体存活性，并为Myostation-intact使用者提供了有关离体肌肉收缩性研究的指导。
