Abstract：
The pharmacodynamic substances of traditional Chinese medicine(TCM) are the basis for the research of TCM and the development of innovative drugs. However, the lack of clarity of targets and molecular mechanisms is the bottleneck problem that restricts the research of pharmacodynamic substances of TCM. Bioactive components are the material basis of the efficacy of TCM, which exert activity by regulating the corresponding targets. Therefore, it is very important to identify the targets of the bioactive components to elucidate the pharmacological mechanism of TCM. Proteins are the most important drug targets, and study of the interaction between the proteins and bioactive components of TCM plays a key role in the development of pharmacological mechanism of TCM. In recent years, the main techniques for detecting the interaction between the bioactive components and proteins include surface plasmon resonance, fluorescence resonance energy transfer, bio-layer interference, molecular docking, proteome chip, target fishing, target mutant, and protein crystallization techniques, etc. This review summarized the biological target detection techniques and their applications in locating the targets of the bioactive components in TCM in the last decade, and this paper will provide useful strategies to elucidate the pharmacological mechanisms of TCM.
摘要：
中药药效物质是中药研究和创新药物开发的基础。然而,作用靶点和分子机制的不明确是制约中药药效物质研究的瓶颈问题。生物活性成分是中药疗效的物质基础,通过调节相应的目标来发挥活性。因此,确定生物活性成分的作用靶点对阐明中药的药理作用机制具有重要意义。蛋白质是最重要的药物靶点，研究中药蛋白质与生物活性成分之间的相互作用对中药药理机制的发展起着关键作用。近年来,检测生物活性成分与蛋白质相互作用的主要技术包括表面等离子体共振,荧光共振能量转移，生物层干扰，分子对接,蛋白组芯片,目标捕鱼,目标突变体,和蛋白质结晶技术，等。本文综述了近十年来生物靶标检测技术及其在中药生物活性成分靶标定位中的应用。本文将为阐明中药的药理机制提供有用的策略。
