PDF(Pubmed)
Abstract：
Chronic kidney disease (CKD) is a global disease that is harder to treat at a later stage. Therefore, early diagnosis and monitoring of CKD are crucial. T cell immunoglobulin and mucin domain molecule 3 (Tim-3) is a negative regulator of the T cell responses and it is involved in the immunomodulation of kidney disease. To date, only a small number of reports regarding serum soluble Tim-3 (sTim-3) in CKD are available. In the present study, the serum levels of sTim-3 in patients with CKD at different stages and the levels of sTim-3 in the early diagnosis and monitoring of CKD were analyzed. A highly sensitive time-resolved fluorescence immunoassay was performed to quantify sTim-3 levels in 318 patients with CKD and 114 healthy individuals. The serum levels of sTim-3 in patients with CKD (33.47±20.77 ng/ml) were significantly higher than those in the healthy individuals group (8.32±3.23 ng/ml; P<0.0001). As CKD progressed from stage G1 to G5, the serum sTim-3 level gradually increased (P<0.0001). A cut-off value of 13.63 ng/ml for the sTim-3 concentration was effective in diagnosing patients with CKD (area under the receiver operating characteristic curve, 0.9176; sensitivity, 79.87%; specificity, 96.49%). At this critical value, the positive detection rate of CKD in the early stages (G1 + G2), G3, G4 and G5 was 55.70, 77.78, 84.44 and 92.86%, respectively. In conclusion, the serum sTim-3 levels in patients with CKD were significantly higher than those in the healthy individuals group. As CKD progressed from G1 to G5, the serum sTim-3 concentration gradually increased, facilitating the monitoring of the progression of CKD. In addition, serum sTim-3 had an auxiliary effect that was useful in the early diagnosis of CKD. The positive detection rate of CKD in the early stages was 55.70%, which can assist other clinically common kidney disease indicators.
摘要：
慢性肾脏病（CKD）是一种全球性疾病，在后期很难治疗。因此,CKD的早期诊断和监测至关重要。T细胞免疫球蛋白和粘蛋白结构域分子3(Tim-3)是T细胞应答的负调节因子,它参与肾脏疾病的免疫调节。迄今为止,只有少数关于CKD血清可溶性Tim-3(sTim-3)的报道。在本研究中,分析不同分期CKD患者血清sTim-3水平及sTim-3水平在CKD早期诊断和监测中的意义。进行了高度灵敏的时间分辨荧光免疫测定，以定量318名CKD患者和114名健康个体的sTim-3水平。CKD患者血清sTim-3水平（33.47±20.77ng/ml）明显高于健康个体组（8.32±3.23ng/ml；P<0.0001）。随着CKD从G1期进展到G5期,血清sTim-3水平逐渐升高(P<0.0001)。sTim-3浓度的临界值为13.63ng/ml可有效诊断CKD患者（受试者工作特征曲线下面积，0.9176；灵敏度，79.87%；特异性，96.49%）。在这个临界值下，早期CKD的阳性检出率（G1+G2），G3、G4和G5分别为55.70、77.78、84.44和92.86%，分别。总之,CKD患者血清sTim-3水平明显高于健康个体组。随着CKD从G1进展到G5,血清sTim-3浓度逐渐升高,促进CKD进展的监测。此外,血清sTim-3对CKD的早期诊断有辅助作用。早期CKD的阳性检出率为55.70%,这可以辅助其他临床上常见的肾脏疾病指标。
