PDF(Pubmed)
Abstract：
Many skin diseases, such as atopic dermatitis (AD), are featured with the dysbiosis of skin microbiota. The clinically recommended options for AD treatments suffer from poor outcomes and high side-effects, leading to severe quality-of-life impairment. To deal with this long-term challenge, we develop a living bacterial formulation (Hy@Rm) that integrates skin symbiotic bacteria of Roseomonas mucosa with poly(vinyl pyrrolidone), poly(vinyl alcohol) and sodium alginate into a skin dressing by virtue of the Ca2+-mediated cross-linking and the freezing-thawing (F-T) cycle method. Hy@Rm dressing creates a favorable condition to not only serve as extrinsic culture harbors but also as nutrient suppliers to support R. mucosa survival in the harsh microenvironment of AD sites to defeat S. aureus, which predominantly colonizes AD skins as an indigenous pathogen, mainly through the secretion of sphingolipids metabolites by R. mucosa like a therapeutics bio-factory. Meanwhile, this elaborately designed skin dressing could accelerate wound healing, normalize aberrant skin characters, recover skin barrier functions, alleviate AD-associated immune/inflammation responses, functioning like a combinational therapy. This study offers a promising means for the topical bacteria transplant to realize effective microbe biotherapy toward the skin diseases feature with microbe milieu disorders, including but not limited to AD disease.
摘要：
许多皮肤病,如特应性皮炎（AD），具有皮肤微生物群的生态失调。临床推荐的AD治疗方案有不良的结果和高的副作用，导致严重的生活质量损害。为了应对这一长期挑战，我们开发了一种活的细菌制剂（Hy@Rm），将玫瑰单胞菌粘膜的皮肤共生细菌与聚（乙烯基吡咯烷酮）整合在一起，通过Ca2介导的交联和冻融（F-T）循环方法，将聚（乙烯醇）和藻酸钠制成皮肤敷料。Hy@Rm敷料创造了一个有利条件，不仅可以作为外在培养港，而且可以作为营养供应商，支持R.粘膜在AD位点恶劣的微环境中存活，以击败金黄色葡萄球菌，主要将AD皮肤作为一种本土病原体定植，主要通过R.粘膜分泌鞘脂代谢产物，如治疗生物工厂。同时,这种精心设计的皮肤敷料可以加速伤口愈合，正常化异常的皮肤特征，恢复皮肤屏障功能，缓解AD相关的免疫/炎症反应，像组合疗法一样运作.本研究为局部细菌移植提供了一种有希望的方法，以实现针对微生物环境紊乱的皮肤病特征的有效微生物生物治疗，包括但不限于AD疾病。
