Abstract：
The quality of many freshwater environments is impacted by human activities, so that many rivers may represent a vehicle for the transmission of health-related microorganisms. This work aimed to isolate and identify genetically free-living amoeba (FLA) of the genus Acanthamoeba from a recreational river in Salta, Argentina, and isolate, if possible, an endocytobiont. Sampling took place at four points (P1-P4) throughout the river in the winter and the summer seasons. Free-living amoebae and Acanthamoeba were recovered from 20-L water concentrated through an ultrafiltration system. Isolation was performed in agar plates, confirmation of Acanthamoeba genus by PCR, and fellow identification and classification based on their sequence analyses. High concentrations of indicator bacteria were found especially in P2, which is intensively used for recreation. Out of a total of 29 FLA isolations, 9 were identified as Acanthamoeba genotype T4 subtype A, the most frequent genotype found in nature and associated with causing human disease. From an axenic culture of Acanthamoeba spp. (KY751412), a bacterial endocytobiont was isolated, and identified as Stenotrophomonas maltophilia. The endocytobiont showed resistance and intermediate resistance to a wide range of widely used antibiotics. Results were in concordance with the cosmopolitan behavior of Acanthamoeba, and showed the importance of studying this group of amoebae and related microorganisms in recreational environments.
摘要：
许多淡水环境的质量受到人类活动的影响，因此，许多河流可能是传播与健康相关的微生物的载体。这项工作旨在从萨尔塔的一条休闲河流中分离和鉴定棘阿米巴属的基因自由生活变形虫（FLA），阿根廷,孤立,如果可能,一个内孢子虫.在冬季和夏季，在整个河流的四个点（P1-P4）进行采样。从通过超滤系统浓缩的20L水中回收自由生活的变形虫和棘阿米巴。在琼脂平板上进行分离，通过PCR确认棘阿米巴属，以及基于序列分析的同伴识别和分类。特别是在P2中发现了高浓度的指示细菌，P2被广泛用于娱乐。在总共29个FLA隔离中，9个被鉴定为棘阿米巴基因型T4亚型A，在自然界中发现的最常见的基因型，与引起人类疾病有关。来自棘阿米巴属的无菌培养物。（KY751412），分离出了一个细菌胞吞生物,并鉴定为嗜麦芽窄食单胞菌。胞吞生物对广泛使用的抗生素表现出抗性和中等抗性。结果与棘阿米巴的世界性行为一致，并显示了在娱乐环境中研究这组变形虫和相关微生物的重要性。
