Abstract：
Infectious bronchitis virus (IBV) is a highly variable RNA virus that affects chickens worldwide. Due to its inherited tendency to suffer point mutations and recombination events during viral replication, emergent IBV strains have been linked to nephropathogenic and reproductive disease that are more severe than typical respiratory disease, leading, in some cases, to mortality, severe production losses, and/or unsuccessful vaccination. QX and DMV/1639 strains are examples of the above-mentioned IBV evolutionary pathway and clinical outcome. In this study, our purpose was to systematically compare whole genomes of QX and DMV strains looking at each IBV gene individually. Phylogenetic analyses and amino acid site searches were performed in datasets obtained from GenBank accounting for all IBV genes and using our own relevant sequences as a basis. The QX dataset studied is more genetically diverse than the DMV dataset, partially due to the greater epidemiological diversity within the five QX strains used as a basis compared to the four DMV strains from our study. Historically, QX strains have emerged and spread earlier than DMV strains in Europe and Asia. Consequently, there are more QX sequences deposited in GenBank than DMV strains, assisting in the identification of a larger pool of QX strains. It is likely that a similar evolutionary pattern will be observed among DMV strains as they develop and spread in North America.
摘要：
传染性支气管炎病毒（IBV）是一种高度可变的RNA病毒，影响全世界的鸡。由于其遗传倾向于在病毒复制过程中遭受点突变和重组事件，新兴的IBV菌株与比典型的呼吸道疾病更严重的肾病和生殖疾病有关，领导,在某些情况下,死亡率，严重的生产损失，和/或不成功的疫苗接种。QX和DMV/1639菌株是上述IBV进化途径和临床结果的实例。在这项研究中,我们的目的是系统地比较QX和DMV毒株的全基因组,分别观察每个IBV基因.在从GenBank获得的数据集中进行系统发育分析和氨基酸位点搜索,所述数据集考虑所有IBV基因并使用我们自己的相关序列作为基础。所研究的QX数据集比DMV数据集更具遗传多样性，部分原因是与我们研究中的4株DMV菌株相比，用作基础的5株QX菌株具有更大的流行病学多样性。历史上,在欧洲和亚洲，QX菌株比DMV菌株更早出现和传播。因此,有更多的QX序列保存在GenBank比DMV菌株，协助鉴定更大的QX菌株库。当DMV毒株在北美发展和传播时，可能会观察到类似的进化模式。
