Abstract：
Women with overactive bladder syndrome (OAB) have a lower urinary ratio of nerve growth factor (NGF) to its precursor (proNGF) compared to healthy controls. MicroRNAs related to NGF and proNGF metabolism and to their receptors may be present in urine and may possess diagnostic value. Urine and blood samples from 20 control and 20 OAB women (50-80 years) were obtained, together with validated questionnaires and other clinical parameters. The relative expression of urinary microRNAs was measured with RT-qPCR. MiR-491-5p, which negatively controls the translation of the matrix metalloproteinase-9 (MMP-9), the main enzyme degrading NGF, was significantly decreased in OAB. Similarly, miR-592, which represses p75NTR receptor synthesis, was down-regulated in OAB. Age, renal function and insulin resistance did not affect these results. ROC curves confirmed the high sensitivity of miR-491-5p and miR-592 for diagnosis. On the other hand, miRNAs involved in the expression of proNGF, of survival receptor TrkA and of markers of nerve integrity were similar between groups. The detection of miR-491-5p and miR-592 in urine could be a useful and non-invasive tool for the diagnosis of OAB syndrome in aging women.
摘要：
与健康对照组相比，患有膀胱过度活动症（OAB）的女性的尿神经生长因子（NGF）与其前体（proNGF）的比例较低。与NGF和proNGF代谢相关的微小RNA及其受体可能存在于尿液中，并可能具有诊断价值。从20名对照和20名OAB妇女（50-80岁）的尿液和血液样本中获得，以及经过验证的问卷和其他临床参数。用RT-qPCR测量尿微小RNA的相对表达。MiR-491-5p,负控制基质金属蛋白酶-9（MMP-9）的翻译，降解NGF的主要酶,在OAB中显著下降。同样，miR-592，抑制p75NTR受体合成，在OAB中下调。年龄,肾功能和胰岛素抵抗不影响这些结果.ROC曲线证实miR-491-5p和miR-592用于诊断的高敏感性。另一方面,参与proNGF表达的miRNA,存活受体TrkA和神经完整性标志物在组间相似.尿液中miR-491-5p和miR-592的检测可能是诊断老年女性OAB综合征的有用和非侵入性工具。
