PDF(Pubmed)
Abstract：
Fecal proteomics allows for the identification of proteins and peptides present in stools and is useful in finding possible new biomarkers for diagnosing and/or monitoring gastrointestinal (GI) disorders. In the present study, we investigated the fecal proteome in healthy and diseased cheetahs (Acinonyx jubatus). Captive individuals of this species frequently show gastrointestinal disorders characterized by recurrent episodes of diarrhea, rare episodes of vomiting and weight loss, associated with Helicobacter spp. infection. Fecal proteomic evaluation has been performed by two-dimensional electrophoresis followed by liquid chromatography-tandem mass spectrometry. In healthy cheetahs, the results showed the presence of the following proteins: collagen alpha-1 (II) chain, transthyretin, IgG Fc-binding protein, titin, dystonin, isopentenyl-diphosphate Delta-isomerase 1, sodium/potassium-transporting ATPase subunit alpha-1 and protein disulfide-isomerase A6. The presence of albumin isoforms was found only in diseased cheetahs. The present paper reports the study of the fecal proteome in the cheetah, evidences some differences between healthy and diseased patients and confirms, once again, the potential of fecal proteomics for the study of the GI environment, with promising developments regarding the identification of new diagnostic/monitoring markers.
摘要：
粪便蛋白质组学允许鉴定粪便中存在的蛋白质和肽,并且可用于发现用于诊断和/或监测胃肠(GI)病症的可能的新生物标志物。在本研究中,我们调查了健康和患病猎豹（Acinonyxjubatus）的粪便蛋白质组。该物种的圈养个体经常表现出胃肠道疾病，其特征是反复发作的腹泻。罕见的呕吐和体重减轻，与螺杆菌有关。感染。粪便蛋白质组学评估已通过双向电泳，然后进行液相色谱-串联质谱。在健康的猎豹中，结果显示存在以下蛋白质:胶原蛋白α-1(II)链,转甲状腺素蛋白，IgGFc结合蛋白，替丁,肌张力素,异戊烯基-二磷酸δ异构酶1，钠/钾转运ATPase亚基α-1和蛋白质二硫键异构酶A6。仅在患病的猎豹中发现白蛋白同工型的存在。本文报道了猎豹粪便蛋白质组的研究，证明了健康患者和患病患者之间的一些差异，并证实了，再一次,粪便蛋白质组学在胃肠道环境研究中的潜力，在鉴定新的诊断/监测标志物方面取得了有希望的进展。
