Abstract：
A new AAPH-Incubating HPLC-DAD-HR MS/MS method was developed for the rapid and high-throughput screening of antioxidants directly in natural products and applied to Gardenia jasminoides fruit. This method was assumed that the peak areas of compounds with potential antioxidant activity in HPLC chromatograms would be significantly reduced or disappeared after incubating with the AAPH which can release ROO at physiological conditions (37 °C, pH 7.4). Additionally, the activity of antioxidants can be evaluated by comparing the peak reduction rates and the screened components can be further identified by HRMS/MS. Then, 17 potential natural antioxidants from the crude extract of GJF was screened. Among them, three major components including crocin I, crocin II and crocetin showed excellent ROO scavenging activity, which were further validated by the ORAC assay. In conclusion, our study provided a simple and effective strategy to rapidly screen antioxidants in natural products.
摘要：
开发了一种新的AAPH-孵育HPLC-DAD-HRMS/MS方法，用于直接在天然产物中快速，高通量地筛选抗氧化剂，并将其应用于栀子果实。该方法被认为在HPLC色谱图上具有潜在抗氧化活性的化合物的峰面积在与能在生理条件(37°C,pH7.4)。此外,抗氧化剂的活性可以通过比较峰值降低率来评估，筛选的成分可以通过HRMS/MS进一步鉴定。然后，从GJF的粗提物中筛选出17种潜在的天然抗氧化剂。其中,三个主要成分，包括crocinI，藏红花素II和藏红花素显示出优异的ROO清除活性，通过ORAC分析进一步验证。总之,我们的研究为快速筛选天然产品中的抗氧化剂提供了一种简单有效的策略.
