Abstract：
Haemonchus contortus (H. contortus) has caused a huge impact on the animal husbandry economy in the world\'s tropical and subtropical regions. Innate immunity is the first-line of host defence. The host recognizes pathogen-associated molecular patterns (PAMPs) through a variety of pattern recognition receptors (PRRs) and activates downstream signalling pathways to resist pathogens invasion. Therefore, elucidating the immune interaction between host and pathogen is key to understanding how the host resists the pathogen. We identified 1516 protein-protein interactions (PPIs) between goat innate immune signal pathway proteins and H. contortus excretory-secretory proteins (ESPs) by Recombination-based \"Library vs. Library\" yeast two-hybrid system (RLL-Y2H) and constructed the PPIs network. Among them, the NLR and IL-17 signalling pathways have the most protein interactions. And there were more interaction proteins between NOD1 and MUC5AC proteins in the pathways. Combined with the differentially expressed genes (DEGs) of susceptible and resistant goats identified in the preliminary work of our laboratory, we selected the intersection genes to construct the PPIs network, and TRAF2 appeared as a key protein of goat innate immune signalling pathway. We initially studied the PPIs between goat and H. contortus ESPs, which provides valuable information for better understanding the immune interaction between the goats and the H. contortus.
摘要：
血门畸形(H.contortus）对世界热带和亚热带地区的畜牧业经济造成了巨大影响。先天免疫是宿主防御的第一道防线。宿主通过多种模式识别受体（PRR）识别病原体相关分子模式（PAMP），并激活下游信号通路以抵抗病原体入侵。因此,阐明宿主与病原体之间的免疫相互作用是理解宿主如何抵抗病原体的关键。我们通过基于重组的“文库与重组”鉴定了山羊先天免疫信号通路蛋白与H.contortus排泄分泌蛋白（ESPs）之间的1516种蛋白质-蛋白质相互作用（PPI）。文库“酵母双杂交系统(RLL-Y2H)并构建了PPI网络。其中,NLR和IL-17信号通路具有最多的蛋白质相互作用。在这些通路中，NOD1和MUC5AC蛋白之间存在更多的相互作用蛋白。结合本实验室前期工作中鉴定的易感山羊和抗性山羊的差异表达基因(DEGs),我们选择了交叉基因来构建PPI网络，TRAF2是山羊先天免疫信号通路的关键蛋白。我们最初研究了山羊和H.contortusESP之间的PPI，这为更好地理解山羊和H.contortus之间的免疫相互作用提供了有价值的信息。
