Abstract：
Primordial germ cells develop into oocytes and sperm cells. These cells are useful resources in reproductive biology and regenerative medicine. The mesenchymal stem cells (MSCs) have been examined for in vitro production of primordial germ cell-like cells. This study aimed to summarize the existing protocols for MSCs differentiation into primordial germ cell-like cells (PGLCs). In the limited identified studies, various models of mesenchymal stem cells, including those derived from adipose tissue, bone marrow, and Wharton\'s jelly, have been successfully differentiated into primordial germ cell-like cells. Although the protocols of specification induction are basically very similar, they have been adjusted to the mesenchymal cell type and the species of origin. The availability of MSCs has made it possible to customize conditions for their differentiation into primordial germ cell-like cells in several models, including humans. Refining germ cell-related signaling pathways during induced differentiation of MSCs will help define extension to the protocols for primordial germ cell-like cells production.
摘要：
原始生殖细胞发育成卵母细胞和精子细胞。这些细胞是生殖生物学和再生医学的有用资源。已检查了间充质干细胞（MSC）的原始生殖细胞样细胞的体外生产。本研究旨在总结MSCs分化为原始生殖细胞样细胞（PGLC）的现有方案。在有限的研究中，间充质干细胞的各种模型，包括来自脂肪组织的那些，骨髓,和沃顿的果冻,已成功分化为原始生殖细胞样细胞。尽管规范诱导的协议基本上非常相似，它们已根据间充质细胞类型和起源物种进行了调整。MSC的可用性使得在几种模型中定制其分化为原始生殖细胞样细胞的条件成为可能。包括人类。在MSC的诱导分化期间完善生殖细胞相关的信号传导途径将有助于定义原始生殖细胞样细胞产生的方案的延伸。
