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Abstract：
Quercetin has a wide range of biological properties that can be used to prevent or decrease particular inflammatory diseases. In this study, we aimed to investigate the gene expression profile and metabolic pathway of the gut microbiota of an antibiotic-treated mouse model administered quercetin. Blood, feces, and intestinal tissue samples were collected and metagenomic sequencing, enzyme-linked immunosorbent assay, and western blot analysis were used to detect variations. The results showed that the quercetin-treated group exhibited increased levels of health beneficial bacterial species, including Faecalibaculum rodentium (103.13%), Enterorhabdus caecimuris (4.13%), Eggerthella lenta (4%), Roseburia hominis (1.33%), and Enterorhabdus mucosicola (1.79%), compared with the model group. These bacterial species were positively related to butyrate, propionate, and intestinal tight junction proteins (zonula occludens-1 and occludin) expression, but negatively related to serum lipopolysaccharide and tumor necrosis factor-α level. In addition, the metabolic pathway analysis showed that dietary quercetin significantly enhanced spliceosomes (111.11%), tight junctions (62.96%), the citrate cycle (10.41%), pyruvate metabolism (6.95%), and lysine biosynthesis (5.06%), but decreasing fatty acid biosynthesis (23.91%) and N-glycan (7.37%) biosynthesis. Furthermore, these metabolic pathway changes were related to relative changes in the abundance of 10 Kyoto Encyclopedia of Genes and Genomes genes (K00244, K00341, K02946, K03737, K01885, k10352, k11717, k10532, K02078, K01191). In conclusion, dietary quercetin increased butyrate-producing bacterial species, and the acetyl-CoA-mediated increased butyrate accelerated carbohydrate, energy metabolism, reduced cell motility and endotoxemia, and increased the gut barrier function, thereby leading to healthy colonic conditions for the host.
摘要：
槲皮素具有广泛的生物学特性，可用于预防或减少特定的炎性疾病。在这项研究中,我们的目的是研究给予槲皮素的抗生素治疗小鼠模型的肠道菌群的基因表达谱和代谢途径。血，粪便,收集肠组织样本并进行宏基因组测序，酶联免疫吸附测定，和蛋白质印迹分析用于检测变异。结果表明，槲皮素治疗组表现出增加的健康有益细菌种类的水平，包括鼠粪杆菌（103.13%），肠背盲肠(4.13%),LentaEggerthella(4%)，人源玫瑰(1.33%),和粘膜肠横纹肌(1.79%)，与模型组相比。这些细菌种类与丁酸盐呈正相关，丙酸盐,和肠紧密连接蛋白（闭合带1和闭合蛋白）的表达，但与血清脂多糖和肿瘤坏死因子-α水平呈负相关。此外,代谢途径分析表明,饲粮槲皮素显著增强剪小体(111.11%),紧密连接（62.96%），柠檬酸盐周期（10.41%），丙酮酸代谢（6.95%），和赖氨酸生物合成（5.06%），但降低脂肪酸的生物合成(23.91%)和N-聚糖的生物合成(7.37%)。此外，这些代谢途径的变化与10个京都百科全书基因和基因组基因(K00244、K00341、K02946、K03737、K01885、k10352、k11717、k10532、K02078、K01191)丰度的相对变化有关。总之,膳食槲皮素增加产生丁酸的细菌种类，乙酰辅酶A介导的丁酸盐加速碳水化合物的增加，能量代谢，细胞运动减少和内毒素血症，增加了肠道屏障功能，从而导致宿主的健康结肠条件。
