Abstract：
Bacterial fermentation of undigested carbohydrates in the hindgut has considerable potential for the stimulation or inhibition of the growth of distinct bacteria within microbiota. The aim of the present study was to evaluate whether high levels of rye affect porcine gut microbiota composition with subsequent effects on the load of Salmonella Typhimurium, an intestinal pathogen with zoonotic relevance. Therefore, forty-two 25-day-old piglets were allocated to two groups and fed a diet containing either 69% wheat or 69% rye for 35 days. One week after introducing the two different diets, the piglets were experimentally infected with Salmonella Typhimurium. The microbiota composition of cecal and fecal samples of the piglets were evaluated 28 days after infection. In the cecum, promoted growth of Bifidobacterium, several lactic acid bacteria and Faecalibacterium prausnitzii were seen in pigs fed the diet containing 69% rye. Bacterial species belonging to the genera Bifidobacterium and Catenisphaera were associated with differing bacterial counts of Salmonella Typhimurium detected in the cecal contents of all piglets in both feeding groups via cultural cultivation. The high intake of rye instead of wheat seems to promote the growth of beneficial intestinal bacteria accompanied by impaired growth conditions for the foodborne pathogen Salmonella Typhimurium.
摘要：
未消化的碳水化合物在后肠中的细菌发酵对于刺激或抑制微生物群内不同细菌的生长具有相当大的潜力。本研究的目的是评估高水平的黑麦是否会影响猪肠道菌群组成，并随后对鼠伤寒沙门氏菌的负荷产生影响，一种与人畜共患有关的肠道病原体。因此,将42只25日龄仔猪分为两组，饲喂含有69％小麦或69％黑麦的饮食35天。在介绍两种不同的饮食一周后，仔猪被实验性感染了鼠伤寒沙门氏菌。感染后28天评估仔猪盲肠和粪便样品的微生物群组成。在盲肠，促进双歧杆菌的生长，在饲喂含69％黑麦的饮食的猪中发现了几种乳酸菌和普氏粪杆菌。属于双歧杆菌属和Catenisphaera属的细菌物种与通过文化培养在两个饲喂组的所有仔猪的盲肠内容物中检测到的鼠伤寒沙门氏菌的不同细菌计数有关。黑麦而不是小麦的高摄入量似乎促进了有益肠道细菌的生长，并伴随着食源性病原体鼠伤寒沙门氏菌的生长条件受损。
