Abstract：
The paper presents the optimization of diode laser irradiation of corroded dental implants in order to reduce the number of microorganisms associated peri-implantitis. The research included the identification of microorganisms on the surface of removed dental implants in patients with peri-implantitis and the assessment of the biocidal effectiveness of the diode laser against these microorganisms. Laser desorption/mass spectrometry (MALDI-TOF MS) was used to identify microorganisms and metagens were examined by next generation sequencing (NGS). Irradiation was performed with a diode laser with a wavelength of λ = 810, operating mode: 25 W/15.000 Hz/10 μs, average = 3.84 W with the number of repetitions t = 2 × 15 s and t = 3 × 15 s. The structure and surface roughness of the implants were analysed before and after laser irradiation by optical profilometry and optical microscopy with confocal fixation. In total, 16 species of Gram-positive bacteria and 23 species of Gram-negative bacteria were identified on the surface of the implants. A total of 25 species of anaerobic bacteria and 12 species with corrosive potential were detected. After diode laser irradiation, the reduction in bacteria on the implants ranged from 88.85% to 100%, and the reduction in fungi from 87.75% to 96.77%. The reduction in microorganisms in the abutment was greater than in the endosseous fixture. The applied laser doses did not damage, but only cleaned the surface of the titanium implants. After 8 years of embedding, the removed titanium implant showed greater roughness than the 25-year-old implant, which was not exposed to direct influence of the oral cavity environment. The use of a diode laser in an optimised irradiation dose safely reduces the number of microorganisms identified on corroded dental implants in patients with peri-implantitis.
摘要：
本文提出了对腐蚀的牙种植体进行二极管激光照射的优化，以减少与种植体周围炎相关的微生物数量。研究包括鉴定种植体周围炎患者去除的牙种植体表面上的微生物，以及评估二极管激光对这些微生物的杀生物效果。使用激光解吸/质谱(MALDI-TOFMS)来鉴定微生物,并且通过下一代测序(NGS)来检查metagen。使用波长为λ=810的二极管激光器进行辐照，工作模式：25W/15.000Hz/10μs，平均=3.84W，重复次数t=2×15s和t=3×15s。通过光学轮廓术和共聚焦固定的光学显微镜分析了激光照射前后植入物的结构和表面粗糙度。总的来说,在植入物的表面上鉴定了16种革兰氏阳性菌和23种革兰氏阴性菌。共检测出25种厌氧菌和12种具有腐蚀潜力的菌。二极管激光辐照后，植入物上细菌的减少范围从88.85%到100%，真菌从87.75%减少到96.77%。基牙中微生物的减少量大于骨内固定装置。施加的激光剂量没有损坏，但只清洁了钛植入物的表面。经过8年的嵌入，移除的钛植入物比25岁的植入物显示出更大的粗糙度，没有受到口腔环境的直接影响。在优化的照射剂量下使用二极管激光器可以安全地减少种植体周围炎患者在腐蚀的牙种植体上鉴定出的微生物数量。
