Abstract：
Representatives of the genus Thiothrix are filamentous, sulfur-oxidizing bacteria found in flowing waters with counter-oriented sulfide and oxygen gradients. They were first described at the end of the 19th century, but the first pure cultures of this species only became available 100 years later. An increase in the number of described Thiothrix species at the beginning of the 21st century shows that the classical phylogenetic marker, 16S rRNA gene, is not informative for species differentiation, which is possible based on genome analysis. Pangenome analysis of the genus Thiothrix showed that the core genome includes genes for dissimilatory sulfur metabolism and central metabolic pathways, namely the Krebs cycle, Embden-Meyerhof-Parnas pathway, glyoxylate cycle, Calvin-Benson-Bassham cycle, and genes for phosphorus metabolism and amination. The shell part of the pangenome includes genes for dissimilatory nitrogen metabolism and nitrogen fixation, for respiration with thiosulfate. The dispensable genome comprises genes predicted to encode mainly hypothetical proteins, transporters, transcription regulators, methyltransferases, transposases, and toxin-antitoxin systems.
摘要：
Thiothrix属的代表是丝状的，在具有反向硫化物和氧气梯度的流动水中发现的硫氧化细菌。它们最早是在19世纪末被描述的，但是这个物种的第一批纯文化直到100年后才出现。21世纪初描述的Thiothrix物种数量的增加表明，经典的系统发育标记，16SrRNA基因，没有提供物种分化的信息，基于基因组分析，这是可能的。Thiothrix属的Pangenome分析表明，核心基因组包括异化硫代谢和中心代谢途径的基因，即克雷布斯循环，Embden-Meyerhof-Parnas通路,乙醛酸循环，卡尔文-本森-巴斯舍姆循环,以及磷代谢和胺化的基因。pangenome的壳部分包括异化氮代谢和固氮的基因，用硫代硫酸盐呼吸。可有可无的基因组包含预测主要编码假设蛋白质的基因，运输商,转录调节剂，甲基转移酶，转座酶,和毒素-抗毒素系统。
