Abstract：
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has led to the most severe global pandemic, which began in Wuhan, China. Angiotensin-converting enzyme 2 (ACE2) combines with the spike protein of SARS-CoV-2, allowing the virus to cross the membrane and enter the cell. SARS-CoV-2 is modified by the transmembrane protease serine 2 (TMPRSS2) to facilitate access to cells. Accordingly, ACE2 and TMPRSS2 are targets of vital importance for the avoidance of SARS-CoV-2 infection. Sanghuangporus sanghuang (SS) is a traditional Chinese medicine that has been demonstrated to have antitumor, antioxidant, anti-inflammatory, antidiabetic, hepatoprotective, neuroprotective and immunomodulatory properties. In this paper, we demonstrated that SS decreased ACE2 and TMPRSS2 expression in cell lines and a mouse model without cytotoxicity or organ damage. Liver and kidney sections were confirmed to have reduced expression of ACE2 and TMPRSS2 by immunohistochemistry (IHC) assessment. Then, hispidin, DBA, PAC, PAD and CA, phenolic compounds of SS, were also tested and verified to reduce the expression of ACE2 and TMPRSS2. In summary, the results indicate that SS and its phenolic compounds have latent capacity for preventing SARS-CoV-2 infection in the future.
摘要：
2019年冠状病毒病（COVID-19）是由严重急性呼吸道综合症冠状病毒2（SARS-CoV-2）引起的，并导致了最严重的全球大流行，始于武汉，中国。血管紧张素转换酶2（ACE2）与SARS-CoV-2的刺突蛋白结合，使病毒穿过膜进入细胞。SARS-CoV-2被跨膜蛋白酶丝氨酸2（TMPRSS2）修饰，以促进进入细胞。因此，ACE2和TMPRSS2是避免SARS-CoV-2感染的重要目标。三黄(SS)是一种已被证明具有抗肿瘤作用的中药,抗氧化剂，抗炎,抗糖尿病药,保肝,神经保护和免疫调节特性。在本文中,我们证明SS降低了细胞系和小鼠模型中ACE2和TMPRSS2的表达，没有细胞毒性或器官损伤。通过免疫组织化学(IHC)评估证实肝切片和肾切片具有降低的ACE2和TMPRSS2表达。然后，Hispidin,DBA,PAC,PAD和CA,SS的酚类化合物，还测试并验证了降低ACE2和TMPRSS2的表达。总之,结果表明，SS及其酚类化合物具有潜在的预防SARS-CoV-2感染的能力。
