Abstract：
Some types of chromosome aberrations are not easily identified by the traditional Giemsa staining. It usually needs some banding technique and skilled person\'s eye. Whole chromosome painting FISH probe can stain designated entire chromosomes or domains in metaphase chromosomes or interphase nuclei, respectively. It allows to visually identify translocations, deletions, or amplifications of specific chromosomes. Once chromosomes are stained, even non-skilled researchers can easily identify those chromosome aberrations. Whole chromosome painting FISH has higher sensitivity to detect chromosome aberrations. This chapter introduces the method for whole chromosome painting FISH staining.
摘要：
传统的Giemsa染色不易识别某些类型的染色体畸变。它通常需要一些条带技术和熟练的人的眼睛。全染色体涂漆FISH探针可以染色指定的整个染色体或中期染色体或间期核的结构域，分别。它允许在视觉上识别易位，删除,或特定染色体的扩增。一旦染色体被染色，即使是不熟练的研究人员也可以很容易地识别这些染色体畸变。全染色体涂漆FISH对检测染色体畸变有较高的敏感度。本章引见了全染色体绘画FISH染色的办法。
