METHODS: We used an in vivo rat model of graft arteriosclerosis and in vitro PDGF-treated VSMCs and identified the novel VSMC contractile phenotype-related gene Bves using a transcriptomic analysis and literature search. In vitro knockdown and overexpression approaches were used to investigate the mechanisms underlying VSMC phenotypic plasticity. In vivo, VSMC-specific Bves overexpression in rat aortic grafts was generated to assess the physiological function of Bves in neointimal lesion development.
RESULTS: Here, we found that Bves expression was negatively regulated in aortic allografts in vivo and PDGF-treated VSMCs in vitro. The genetic knockdown of Bves dramatically inhibited, whereas Bves overexpression markedly promoted, the VSMC contractile phenotype. Furthermore, RNA sequencing unraveled a positive correlation between Bves and dual-specificity protein phosphatase 1 (Dusp1) expression in VSMCs. We found that Bves knockdown restrained Dusp1 expression, but enhanced p38MAPK and ERK1/2 activation, resulting in the loss of the VSMC contractile phenotype. In vivo, an analysis of a rat graft model confirmed that VSMC-specific Bves and Dusp1 overexpression in aortic allografts significantly attenuated neointimal lesion formation.
CONCLUSIONS: Bves maintains the VSMC contractile phenotype through Dusp1-dependent p38MAPK and ERK1/2 signaling, and protects against neointimal formation, underscoring the important role of Bves in preventing transplant vasculopathy.
方法:我们使用移植物动脉硬化和体外PDGF处理的VSMC的体内大鼠模型,并通过转录组学分析和文献检索鉴定了新的VSMC收缩表型相关基因Bves。使用体外敲低和过表达方法来研究VSMC表型可塑性的潜在机制。在体内,在大鼠主动脉移植物中产生VSMC特异性Bves过表达,以评估Bves在新内膜病变发展中的生理功能。
结果:这里,我们发现Bves的表达在体内主动脉移植物和体外PDGF处理的VSMC中负向调节。Bves的基因敲除显著抑制,而Bves过表达显着促进,VSMC收缩表型。此外,RNA测序揭示了VSMC中Bves与双特异性蛋白磷酸酶1(Dusp1)表达之间的正相关关系。我们发现Bves敲低抑制了Dusp1的表达,但增强p38MAPK和ERK1/2激活,导致VSMC收缩表型的丧失。在体内,对大鼠移植模型的分析证实,主动脉同种异体移植物中VSMC特异性Bves和Dusp1的过表达显着减轻了新内膜病变的形成。
结论:Bves通过Dusp1依赖性p38MAPK和ERK1/2信号维持VSMC收缩表型,并防止新内膜形成,强调Bves在预防移植血管病变中的重要作用。