PDF(Pubmed)
Abstract：
Introduction: The complete inactivation of infectious tissues of large animal carcasses is one of the most challenging tasks in high-containment facilities. Steam sterilization is a method frequently in use to achieve biological inactivation of liquid and solid waste. Objective: This study aims to highlight parameters most effective in creating reproducible cycles for steam sterilization of pig and calf carcasses. Methods: Two pigs or 1 calf were sterilized by running a liquid cycle (n = 3) at 121°C for at least 120 minutes in a pass-through autoclave. To assess the physical and biological parameters, temperature data loggers and biological indicators (BIs) with spores of Geobacillus stearothermophilus (ATCC 7953) were placed at defined positions within animal carcasses. After completion of each cycle, data loggers were analyzed and BIs were incubated for 7 days at 60°C. Results: Initial testing with an undissected pig carcass resulted in suboptimal temperatures at the tissue level with growth on 1 BI. After modifications of the used stainless-steel boxes and by placing the reference probe of the autoclave in the animal carcass, reproducible cycles could be created. A complete inactivation of BIs and a temperature profile of >121°C for at least 20 minutes could be achieved in almost all probed tissues. Conclusion: Only minor modifications in carcass preparation and the used sterilization equipment resulted in effective and reproducible cycles to inactivate large animal carcasses by using a steam autoclave.
摘要：
简介：完全灭活大型动物尸体的感染性组织是高围护设施中最具挑战性的任务之一。蒸汽灭菌是实现液体和固体废物的生物灭活的常用方法。目的：本研究旨在强调在创建猪和小牛尸体蒸汽灭菌的可重复循环中最有效的参数。方法:通过在通过式高压釜中在121°C下运行液体循环(n=3)至少120分钟来对两只猪或1只小牛进行灭菌。为了评估物理和生物学参数，将带有嗜热脂肪土芽孢杆菌（ATCC7953）孢子的温度数据记录器和生物指示器（BI）放置在动物尸体内的指定位置。每个周期完成后，分析数据记录器,并将BIs在60°C下孵育7天。结果：对未解剖的猪car体进行的初始测试导致组织水平的温度欠佳，并在1BI上生长。在对使用过的不锈钢箱进行修改后，并将高压釜的参考探针放置在动物尸体中，可以创建可重复的循环。在几乎所有探测的组织中都可以实现BI的完全失活和>121°C的温度分布持续至少20分钟。结论：仅对car体准备和使用的灭菌设备进行了微小的修改，就可以通过使用蒸汽高压灭菌器来有效且可重复地循环灭活大型动物car体。
