Abstract：
DNA recombination repair systems are essential for organisms to maintain genomic stability. In recent years, we have improved our understanding of the mechanisms of RecBCD/AddAB family-mediated DNA double-strand break repair. In E. coli, it is RecBCD that plays a central role, and in Firmicute Bacillus subtilis it is the AddAB complex that functions. However, there are open questions about the mechanism of DNA repair in bacteria. For example, how bacteria containing crossover hotspot instigator (Chi) sites regulate the activity of proteins. In addition, we still do not know the exact process by which the RecB nuclease or AddA nuclease structural domains load RecA onto DNA. We also know little about the mechanism of DNA repair in the industrially important production bacterium Corynebacterium glutamicum (C. glutamicum). Therefore, exploring DNA repair mechanisms in bacteria may not only deepen our understanding of the DNA repair process in this species but also guide us in the targeted treatment of diseases associated with recombination defects, such as cancer. In this paper, we firstly review the classical proteins RecBCD and AddAB involved in DNA recombination repair, secondly focus on the novel helical nuclease AdnAB found in the genus Mycobacterium.
摘要：
DNA重组修复系统是生物体维持基因组稳定性所必需的。近年来,我们提高了对RecBCD/AddAB家族介导的DNA双链断裂修复机制的认识.在大肠杆菌中，RecBCD发挥了核心作用，在枯草芽孢杆菌中，它是起作用的AddAB复合物。然而,有关于细菌DNA修复机制的公开问题。例如,含有交叉热点煽动者（Chi）位点的细菌如何调节蛋白质的活性。此外,我们仍然不知道RecB核酸酶或AddA核酸酶结构域将RecA加载到DNA上的确切过程。我们对工业上重要的生产细菌谷氨酸棒杆菌（C.谷氨酸）。因此,探索细菌中的DNA修复机制不仅可以加深我们对该物种DNA修复过程的理解，还可以指导我们靶向治疗与重组缺陷相关的疾病，比如癌症。在本文中,本文首先综述了经典的参与DNA重组修复的蛋白质RecBCD和AddAB,其次重点研究了在分枝杆菌属中发现的新型螺旋核酸酶AdnAB。
