Abstract：
The current trend toward using natural food additives, cosmetics, and medicines has motivated industries to substitute synthetic compounds for natural products. Essential oils (EOs) from medicinal plants are a well-known source of chemical compounds that display several interesting biological activities, including antimicrobial action. In this study, we investigated the antibacterial activity of EOs extracted from three Piperaceae species collected in the Brazilian Amazon region against a representative panel of cariogenic bacteria. The minimum inhibitory concentration (MIC) of the essential oils extracted from Peperomia pellucida (PP-EO), Piper marginatum (PM-EO), and Piper callosum (PC-EO) was determined against Streptococcus mutans, S. mitis, S. sanguinis, S. salivarius, S. sobrinus, Enterococcus faecalis, and Lactobacillus casei by using the microplate microdilution method. PM-EO, PC-EO, and PP-EO displayed antibacterial activity against all the tested cariogenic bacteria. PM-EO displayed the best inhibitory activity, with MIC values ranging from 50 to 500 µg/mL. The lowest MIC values were obtained for PM-EO against S. mitis (MIC = 75 μg/mL), Lactobacillus casei (MIC = 50 μg/mL), and S. mutans (MIC = 50 μg/mL). Gas chromatography mass spectrometry (GC-MS) analysis allowed the chemical composition of all the EOs to be identified. The main constituents of PM-EO, PC-EO, and PP-EO were 3,4-(methylenedioxy)propiophenone, α-pinene, and dillapiole, respectively. Finally, the compounds that were exclusively detected in PM-EO are highlighted. Our results suggest that PM-EO may be used in products for treating dental caries and periodontal diseases.
摘要：
目前使用天然食品添加剂的趋势，化妆品,和药物促使工业用合成化合物代替天然产物。来自药用植物的精油（EO）是一种众所周知的化合物来源，显示出几种有趣的生物活性。包括抗菌作用。在这项研究中,我们调查了从巴西亚马逊地区收集的三种胡椒科物种中提取的EO对一组代表性的致龋细菌的抗菌活性。最小抑制浓度（MIC）的精油提取pellucida（PP-EO），边缘吹笛者（PM-EO），和Pipercall（PC-EO）被确定为抗变异链球菌，S.米蒂斯，S、血统，唾液，S.Sobrinus,粪肠球菌,和干酪乳杆菌，采用微孔板微量稀释法。PM-EO,PC-EO,和PP-EO对所有测试的致龋细菌显示出抗菌活性。PM-EO表现出最好的抑制活性,MIC值范围为50至500µg/mL。PM-EO对S.mitis的MIC值最低（MIC=75μg/mL），干酪乳杆菌（MIC=50μg/mL），和变形链球菌(MIC=50μg/mL)。气相色谱质谱(GC-MS)分析允许鉴定所有EO的化学组成。PM-EO的主要成分,PC-EO,和PP-EO是3,4-（亚甲二氧基）苯乙酮，α-pine烯,还有Dillapiole,分别。最后,强调了在PM-EO中专门检测到的化合物。我们的结果表明，PM-EO可用于治疗龋齿和牙周病的产品。
