关键词: Salmonella epidemiology foodborne pathogen pigs pre-harvest real-time PCR serology sows Salmonella epidemiology foodborne pathogen pigs pre-harvest real-time PCR serology sows Salmonella epidemiology foodborne pathogen pigs pre-harvest real-time PCR serology sows

来  源:   DOI:10.3390/microorganisms10081532

Abstract:
In piglet production, the beginning of pork production, Salmonella prevalence requires greater attention as having an impact on the subsequent production steps. The aim of this study was to investigate Salmonella prevalence in three sow herds with attached piglet rearing units. Salmonella prevalence was investigated either directly by boot swabs and feces or indirectly by serum samples taken during gilt integration, the peripartal period, and piglet rearing. Boot swabs and feces were analyzed by real-time PCR and subsequent microbiology. Results indicated that high biosecurity measures in sow husbandry do not necessarily result in a low Salmonella prevalence. Furthermore, the sow herds\' Salmonella prevalence should not be used to infer the situation in the associated piglet rearing. The proportion of positive boot swabs was 10.5, 3.6, and 21.3% for sows (gilts and peripartal) with an inverse situation in piglet rearing with 50.0, 63.3, and 5.8% positive swabs for farms A, B, and C, respectively. Boot swabs are suitable as a direct sampling method to gain an overview of Salmonella prevalence in both sows and piglets. Indirect serum antibody testing can be useful, although it should be evaluated considering age-dependent levels of antibody titres.
摘要:
在仔猪生产中,猪肉生产的开始,沙门氏菌的流行需要更多的关注,因为对随后的生产步骤有影响。这项研究的目的是调查三只母猪中沙门氏菌的流行情况。沙门氏菌的患病率可以直接通过靴子拭子和粪便进行调查,也可以通过在镀金整合期间采集的血清样本进行间接调查。围产期,和仔猪饲养。通过实时PCR和随后的微生物学分析皮靴拭子和粪便。结果表明,母猪饲养中的高生物安全措施并不一定会导致沙门氏菌的低患病率。此外,母猪群沙门氏菌的患病率不应被用来推断相关仔猪饲养的情况。母猪(后备母猪和围产期)的阳性靴拭子比例为10.5、3.6和21.3%,而仔猪饲养的情况相反,农场A的阳性拭子为50.0、63.3和5.8%,B,C,分别。靴子拭子适合作为直接采样方法,以概述母猪和仔猪中沙门氏菌的流行情况。间接血清抗体检测可以是有用的,尽管应该考虑年龄依赖性的抗体滴度水平进行评估。
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