PDF(Pubmed)
Abstract：
The application of lipotransfer after breast-conserving therapy (BCT) and irradiation in breast cancer patients is an already widespread procedure for reconstructing volume deficits of the diseased breast. Nevertheless, the safety of lipotransfer has still not been clarified yet due to contradictory data. The goal of this in vitro study was to further elucidate the potential effects of lipotransfer on the irradiated remaining breast tissue. The mammary epithelial cell line MCF-10A was co-cultured with the fibroblast cell line MRC-5 and irradiated with 2 and 5 Gy. Afterwards, cells were treated with conditioned medium (CM) from adipose-derived stem cells (ADSC), and the effects on the cellular functions of MCF-10A cells and on gene expression at the mRNA level in MCF-10A and MRC-5 cells were analyzed. Treatment with ADSC CM stimulated transmigration and invasion and decreased the surviving fraction of MCF-10A cells. Further, the expression of cytokines, extracellular, and mesenchymal markers was enhanced in mammary epithelial cells. Only an effect of ADSC CM on irradiated fibroblasts could be observed. The present data suggest epithelial-mesenchymal transition-like changes in the epithelial mammary breast cell line. Thus, the benefits of lipotransfer after BCT should be critically weighed against its possible risks for the affected patients.
摘要：
在乳腺癌患者的保乳治疗（BCT）和放疗后应用脂肪转移是重建患病乳房体积不足的广泛方法。然而，由于相互矛盾的数据,脂转移的安全性仍未得到澄清.这项体外研究的目的是进一步阐明脂肪转移对经照射的剩余乳腺组织的潜在影响。将乳腺上皮细胞系MCF-10A与成纤维细胞系MRC-5共培养并用2和5Gy照射。之后,细胞用来自脂肪来源的干细胞（ADSC）的条件培养基（CM）处理，分析了MCF-10A和MRC-5细胞对MCF-10A细胞功能和mRNA水平基因表达的影响。用ADSCCM处理刺激了MCF-10A细胞的迁移和侵袭并降低了存活分数。Further,细胞因子的表达，细胞外，乳腺上皮细胞间充质标志物增强。只能观察到ADSCCM对照射的成纤维细胞的作用。目前的数据表明上皮乳腺细胞系中上皮-间质转化样的变化。因此，应严格权衡BCT后脂肪转运的益处及其对受影响患者的可能风险.
