Abstract:
In order to improve the anticaries effects of fluoridated products, the supplementation of these products has been considered a promising alternative for caries control. This study evaluated the effects of sodium hexametaphosphate (HMP) and/or fluoride (F) on the inorganic components and pH of Streptococcus mutans and Candida albicans dual-species biofilms. The biofilms were treated 72, 78, and 96 h after the beginning of their formation with 0.25, 0.5, or 1% HMP-containing solutions with or without F (500 ppm, as sodium fluoride). F-containing solutions (500 ppm and 1100 ppm) and artificial saliva were used as controls. The biofilms were exposed to a 20% sucrose solution after the third treatment. Along with the biofilm pH, the concentrations of F, calcium, phosphorus (P), and HMP were determined. HMP, combined with F, increased F levels and decreased P levels in the biofilm fluid compared to that of the solution with 500 ppm F. Exposure to sucrose decreased the concentrations of all ions in the biomass, except for HMP; 1% HMP, combined with F, promoted the highest pH. It can be concluded that HMP affected the inorganic composition of the biofilm and exerted a buffering effect on the biofilm pH.
摘要:
为了提高氟化产品的防龋效果,补充这些产品被认为是控制龋齿的有希望的替代方法。这项研究评估了六偏磷酸钠(HMP)和/或氟化物(F)对变形链球菌和白色念珠菌双物种生物膜的无机成分和pH的影响。生物膜在开始形成后72、78和96小时用含有或不含F的0.25、0.5或1%HMP的溶液处理(500ppm,如氟化钠)。使用含F的溶液(500ppm和1100ppm)和人工唾液作为对照。在第三次处理后,将生物膜暴露于20%蔗糖溶液。随着生物膜的pH值,F的浓度,钙,磷(P),确定了HMP。HMP,结合F,与500ppmF的溶液相比,生物膜流体中的F水平增加,P水平降低。暴露于蔗糖会降低生物质中所有离子的浓度,HMP除外;1%HMP,结合F,促进pH最高。可以得出结论,HMP影响生物膜的无机组成并对生物膜的pH具有缓冲作用。