Abstract：
Inflammatory bowel disease (IBD) is a chronic recurrent inflammatory disease with unknown etiology. Dextran sulfate sodium (DSS) induced colitis is a widely used mouse model in IBD research. DSS colitis involves activation of the submucosal immune system and can be used to study IBD-like disease characteristics in acute, chronic, remission and transition phases. Insight into colon inflammatory parameters is needed to understand potentially irreversible adaptations to the chronification of colitis, determining the baseline and impact of further inflammatory episodes. We performed analyses of non-invasive and invasive colitis parameters in acute, chronic and remission phases of the DSS colitis in C57BL/6 mice. Non-invasive colitis parameters poorly reflected inflammatory aspects of colitis in chronic remission phase. We found invasive inflammatory parameters, positively linked to repeated DSS-episodes, such as specific colon weight, inflamed colon area, spleen weight, absolute cell numbers of CD4+ and CD8+ T cells as well as B cells, blood IFN-γ level, colonic chemokines BLC and MDC as well as the prevalence of Turicibacter species in feces. Moreover, microbial Lactobacillus species decreased with chronification of disease. Our data point out indicative parameters of recurrent gut inflammation in context of DSS colitis.
摘要：
炎症性肠病(IBD)是一种病因不明的慢性复发性炎症性疾病。葡聚糖硫酸钠(DSS)诱导的结肠炎是IBD研究中广泛使用的小鼠模型。DSS结肠炎涉及粘膜下免疫系统的激活，可用于研究急性IBD样疾病的特征，慢性，缓解和过渡阶段。需要了解结肠炎症参数，以了解对结肠炎慢性化的潜在不可逆适应，确定基线和进一步炎症发作的影响。我们对急性非侵入性和侵入性结肠炎参数进行了分析，C57BL/6小鼠DSS结肠炎的慢性和缓解期。非侵入性结肠炎参数很少反映慢性缓解期结肠炎的炎症方面。我们发现了侵入性炎症参数，与重复的DSS发作呈正相关，例如特定的结肠重量，发炎的结肠区域，脾脏重量,CD4+和CD8+T细胞以及B细胞的绝对细胞数，血液IFN-γ水平，结肠趋化因子BLC和MDC以及粪便中Turicibacter物种的流行。此外，微生物乳杆菌种类随着疾病的慢性化而减少。我们的数据指出了DSS结肠炎中复发性肠道炎症的指示性参数。
