Abstract：
Superovulation of high-producing dairy cows is a challenging subject in dairy farms with respect to the cost, dose and type of gonadotropin. The objectives of this study were to compare three gonadotropin products: Folltropin-V® (highly purified FSH with porcine origin), Cinnal-f® (recombinant human FSH) and Menotropins® (hMG) for superovulation in high-producing Holstein lactating dairy cows and to investigate the pregnancy outcomes achieved following transferring embryos recovered from donors treated with different gonadotropins. Healthy high-producing Holstein lactating dairy cows (n = 30; milk production: 46.35 ± 8.78 kg; parity: 2-4; days in milk: 80-130 days) without any puerperal problems were selected as donors. On Day 10 after estrus (Day 0 of superovulation), donors (10 cows in each experimental groups) received Folltropin-V® (400 mg NIH, dissolved in 20 ml), Cinnal-f® (20 vials; each vial of 1 ml contains 75 IU Follitropin alfa) and Menotropins ® (20 ampules; each ampule of 1 ml contains 75 IU FSH and 75 IU LH), administered twice daily, in decreasing doses (4,4; 3,3; 2,2; 1,1 ml), over 4 days. On Day 2 of superovulation, donors received 3 doses of prostaglandin F2α analogue, 6 h apart. They were inseminated twice with a frozen semen at 12 and 24 h after standing estrus. Concurrent with the second insemination, donors received 2500 IU hCG (Karma Pharmatech GmbH, Germany). On Day 7 after standing estrus, superovulatory responses (number of CLs, total ova/embryos and transferable embryos) were recorded and Code 1 embryos, recovered from each treated donors, were transferred to synchronized heifers. Pregnancy was detected on Day 30 and 60 after AI. Gestation length, the number and weight of live births were recorded. Data were analyzed using Proc GLM, Proc Mixed and Proc Genmod of SAS. The respective number of corpora lutea, total number of ova/embryos and transferable embryos were not different among donors received Cinnal-f (25.5 ± 3.01, 11.2 ± 2.77, 5.1 ± 0.86), Menotropins (24.0 ± 3.21, 9.0 ± 2.04, 6.3 ± 1.74) and Folltropin-V (20.3 ± 3.21, 8.9 ± 1.90, 5.1 ± 1.16; P > 0.05). Pregnancy rates on Day 30 was similar among treatment groups (P > 0.05). However, pregnancy rates on Day 60 and the number of calves born healthy was less in heifers that received embryos from Cinnal-f treated donors (P < 0.05). In conclusion, Cinnal-f and Menotropins could provide similar superovulatory response to Folltropin-V for superovulation of high-producing Holstein lactating dairy cows.
摘要：
高产奶牛的超数排卵在奶牛场中是一个具有挑战性的课题，促性腺激素的剂量和类型。本研究的目的是比较三种促性腺激素产品：Folltropin-V®（高度纯化的猪来源的FSH），Cinnal-f®（重组人FSH）和Menotropins®（hMG）用于高产荷斯坦泌乳奶牛的超排卵，并研究从接受不同促性腺激素治疗的供体中回收的胚胎移植后获得的妊娠结局。选择健康的高产荷斯坦泌乳奶牛（n=30；产奶量：46.35±8.78kg；胎次：2-4；牛奶天数：80-130天），没有任何产褥期问题。在发情期后的第10天（超排卵的第0天），供体（每个实验组10头母牛）接受了Folltropin-V®（400毫克NIH，溶解在20毫升中)，Cinnal-f®（20个小瓶；每瓶1毫升含有75IUFollitropinalfa）和Menotropins®（20安瓿；每瓶1毫升含有75IUFSH和75IULH），每天服用两次，在递减剂量（4,4;3,3;2,2;1毫升），超过4天。在超数排卵的第二天，供体接受了3剂前列腺素F2α类似物，6h分开。他们在发情期后12和24小时用冷冻精液授精两次。同时进行第二次授精，捐赠者接受了2500IU的hCG(KarmaPharmatechGmbH，德国)。在站立发情后的第7天，超排卵反应(CLs数量，总卵子/胚胎和可转移胚胎)记录和代码1胚胎，从每个接受治疗的捐赠者那里回收，被转移到同步母牛。在AI后第30天和第60天检测到妊娠。妊娠长度,记录了活产婴儿的数量和体重.使用ProcGLM分析数据，SAS的Proc混合和ProcGenmod。相应的黄体数量，接受Cinnal-f（25.5±3.01，11.2±2.77，5.1±0.86）的供体中，卵子/胚胎和可转移胚胎的总数没有差异，月经素（24.0±3.21，9.0±2.04，6.3±1.74）和Folltropin-V（20.3±3.21，8.9±1.90，5.1±1.16；P>0.05）。第30天的妊娠率在治疗组之间相似(P>0.05)。然而,在接受Cinnal-f处理供体胚胎的小母牛中，第60天的妊娠率和健康出生的小牛数量较少（P<0.05）。总之,Cinnal-f和Menotropins可以提供与Folltropin-V相似的超排卵反应，用于高产荷斯坦泌乳奶牛的超排卵。
