Abstract：
This current review is focused on the generation and settled patterns of mouse Purkinje cells (PCs) and deep cerebellar nuclei (DCN) neurons. By mean of progressively delayed comprehensive labeling procedure, I will show, with the technique of [3H] thymidine autoradiography, the quantitative determination of PCs and DCN neurons production along the mediolateral and rostrocaudal axes of the cerebellum. The procedure consists of injecting groups of pregnant mice, on specific embryonic (E) days, with two doses of [3H] thymidine in an overlapping series with 24 h delays between groups (E11-12, E12-13, E13-14, E14-15). The analysis of the autoradiograms revealed that PCs and DCN neurons are sequentially generated following precise neurogenetic timetables. PCs are born somewhat later than the DCN neurons. Both macroneurons are produced following two gradients. The first of these is mediolateral and the second is rostrocaudal. On the other hand, it will be also shown that PCs and DCN neurons were settled in the cerebellum following accurate neurogenetic gradients. These data have suggested that the chronological sequence of neuron production is a key factor in facilitating, in the adulthood, the cytoarchitecture of the cerebellum, and the establishment of patterns of orderly connections between PCs and DCN neurons.
摘要：
当前的评论集中在小鼠浦肯野细胞（PC）和深小脑核（DCN）神经元的生成和稳定模式上。通过逐步延迟的全面标签程序，我会展示,用[3H]胸苷放射自显影技术，沿着小脑的中外侧和前脑轴定量测定PCs和DCN神经元的产生。该程序包括注射怀孕小鼠组，在特定的胚胎（E）天，两个剂量的[3H]胸苷在一个重叠的系列，24小时延迟组（E11-12，E12-13，E13-14，E14-15）。对放射自显影图的分析表明，PC和DCN神经元是按照精确的神经遗传学时间表顺序产生的。PC比DCN神经元稍晚出生。两个马神经元都是按照两个梯度产生的。第一个是中外侧，第二个是房尾。另一方面,它还将显示，PC和DCN神经元在准确的神经遗传梯度后定居在小脑中。这些数据表明，神经元产生的时间顺序是促进，在成年后，小脑的细胞结构，以及PCs和DCN神经元之间有序连接模式的建立。
