Abstract：
BACKGROUND: The aim of this study was to investigate the expression of aquaporin 1 (AQP-1), AQP-3 and vascular endothelial growth factor A (VEGF-A) in peritoneal tissues of patients without kidney disease, chronic kidney disease at stages 5 (CKD 5) and patients on prolonged peritoneal dialysis with ultrafiltration failure (PDUFF), and elucidate the possible mechanism of peritoneal dialysis ultrafiltration failure.
METHODS: Peritoneal specimens were collected from the following patient groups at Xianju People\'s hospital: CKD 5, PD-UFF and normal control groups. Routine staining and immunohistochemical analyses were performed on samples obtained from the three groups.
RESULTS: The expression of AQP-1 and AQP-3 on peritoneal mesothelial cells, peritoneal vessels and in the interstitium was significantly lower in the PD-UFF group than the CKD 5 and control groups (P < .01), while no statistically significant difference was found between the CKD 5 and control groups (P > .05). In contrast, VEGF-A expression was significantly higher in peritoneal mesothelial cells, peritoneal vessels and the interstitium in the PD-UFF group than the CKD 5 and control groups (P < .01). No statistically significant difference was found between the CKD 5 and control groups (P > .05).
CONCLUSIONS: AQP-1 and AQP-3 expression levels decrease in peritoneal mesothelial cells and the vascular interstitium of patients with a prolonged peritoneal dialysis course, while VEGF-A expression gradually increases. The formation of peritoneal neovascularization and the decrease in AQP expression may be primarily associated with peritoneal dialysis ultrafiltration failure. DOI: 10.52547/ijkd.6928.
METHODS: Peritoneal specimens were collected from the following patient groups at Xianju People\'s hospital: CKD 5, PD-UFF and normal control groups. Routine staining and immunohistochemical analyses were performed on samples obtained from the three groups.
RESULTS: The expression of AQP-1 and AQP-3 on peritoneal mesothelial cells, peritoneal vessels and in the interstitium was significantly lower in the PD-UFF group than the CKD 5 and control groups (P < .01), while no statistically significant difference was found between the CKD 5 and control groups (P > .05). In contrast, VEGF-A expression was significantly higher in peritoneal mesothelial cells, peritoneal vessels and the interstitium in the PD-UFF group than the CKD 5 and control groups (P < .01). No statistically significant difference was found between the CKD 5 and control groups (P > .05).
CONCLUSIONS: AQP-1 and AQP-3 expression levels decrease in peritoneal mesothelial cells and the vascular interstitium of patients with a prolonged peritoneal dialysis course, while VEGF-A expression gradually increases. The formation of peritoneal neovascularization and the decrease in AQP expression may be primarily associated with peritoneal dialysis ultrafiltration failure. DOI: 10.52547/ijkd.6928.
摘要：
背景：这项研究的目的是研究水通道蛋白1（AQP-1）的表达，非肾病患者腹膜组织AQP-3和血管内皮生长因子A(VEGF-A),慢性肾脏病5期（CKD5期）和长期腹膜透析伴超滤衰竭（PDUFF）的患者，并阐明腹膜透析超滤失败的可能机制。
方法：从仙居人民医院的以下患者组中收集腹膜标本：CKD5，PD-UFF和正常对照组。对从三组获得的样品进行常规染色和免疫组织化学分析。
结果：AQP-1和AQP-3在腹膜间皮细胞上的表达，PD-UFF组的腹膜血管和间质明显低于CKD5组和对照组（P<0.01），CKD5组与对照组比较差异无统计学意义(P>.05)。相比之下,VEGF-A在腹膜间皮细胞中表达显著增高,PD-UFF组的腹膜血管和间质高于CKD5组和对照组（P<0.01）。CKD5组与对照组比较差异无统计学意义(P>.05)。
结论：腹膜透析病程延长的患者，腹膜间皮素和血管间质中AQP-1和AQP-3的表达水平降低，而VEGF-A表达逐渐增加。腹膜新生血管的形成和AQP表达的降低可能主要与腹膜透析超滤失败有关。DOI：10.52547/ijkd.6928。
方法：从仙居人民医院的以下患者组中收集腹膜标本：CKD5，PD-UFF和正常对照组。对从三组获得的样品进行常规染色和免疫组织化学分析。
结果：AQP-1和AQP-3在腹膜间皮细胞上的表达，PD-UFF组的腹膜血管和间质明显低于CKD5组和对照组（P<0.01），CKD5组与对照组比较差异无统计学意义(P>.05)。相比之下,VEGF-A在腹膜间皮细胞中表达显著增高,PD-UFF组的腹膜血管和间质高于CKD5组和对照组（P<0.01）。CKD5组与对照组比较差异无统计学意义(P>.05)。
结论：腹膜透析病程延长的患者，腹膜间皮素和血管间质中AQP-1和AQP-3的表达水平降低，而VEGF-A表达逐渐增加。腹膜新生血管的形成和AQP表达的降低可能主要与腹膜透析超滤失败有关。DOI：10.52547/ijkd.6928。
