关键词: Biofuel Cellulase Genetic engineering Genomic mining Lignocellulosic biomass Marine actinobacteria

Mesh : Actinobacteria / genetics metabolism Bacteria / metabolism Biofuels Biomass Cellulase / chemistry genetics metabolism Cellulose / metabolism

来  源:   DOI:10.1016/j.envres.2022.114018

Abstract:
Every year, 180 billion tonnes of cellulose are produced by plants as waste biomass after the cultivation of the desired product. One of the smart and effective ways to utilize this biomass rather than burn it is to utilize the biomass to adequately meet the energy needs with the help of microbial cellulase that can catalytically convert the cellulose into simple sugar units. Marine actinobacteria is one of the plentiful gram-positive bacteria known for its industrial application as it can produce multienzyme cellulase with high thermal tolerance, pH stability and high resistant towards metal ions and salt concentration, along with other antimicrobial properties. Highly stable cellulase obtained from marine actinobacteria will convert the cellulose biomass into glucose, which is the precursor for biofuel production. This review will provide a comprehensive outlook of various strategic applications of cellulase from marine actinobacteria which can facilitate the breakdown of lignocellulosic biomass to bioenergy with respect to its characteristics based on the location/environment that the organism was collected and its screening strategies followed by adopted methodologies to mine the novel cellulase genome and enhance the production, thereby increasing the activity of cellulase continued by effective immobilization on novel substrates for the multiple usage of cellulase along with the industrial applications.
摘要:
每年,在培养所需产品后,植物生产了1800亿吨纤维素作为废物生物质。利用这种生物质而不是燃烧它的智能和有效的方法之一是利用生物质在微生物纤维素酶的帮助下充分满足能量需求,所述微生物纤维素酶可以催化地将纤维素转化为简单的糖单元。海洋放线菌是以其工业应用而闻名的大量革兰氏阳性菌之一,因为它可以产生具有高耐热性的多酶纤维素酶,pH稳定性和对金属离子和盐浓度的高抗性,以及其他抗菌性能。从海洋放线菌中获得的高度稳定的纤维素酶将纤维素生物质转化为葡萄糖,这是生物燃料生产的前体。这篇综述将全面展望海洋放线菌纤维素酶的各种战略应用,这些应用可以促进木质纤维素生物质分解为生物能源,其特征是基于生物收集的位置/环境及其筛选策略,然后采用方法来挖掘新的纤维素酶基因组并提高产量,从而通过有效固定在新型底物上继续增加纤维素酶的活性,以实现纤维素酶的多种用途以及工业应用。
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