PDF(Pubmed)
Abstract：
The interaction of SARS-CoV-2 with the human immune system is at the basis of the positive or negative outcome of the infection. Monocytes and macrophages, which are major innate immune/inflammatory effector cells, are not directly infected by SARS-CoV-2, however they can react to the virus and mount a strong reaction. Whether this first interaction and reaction may bias innate reactivity to re-challenge, a phenomenon known as innate memory, is currently unexplored and may be part of the long-term sequelae of COVID-19. Here, we have tested the capacity of SARS-CoV-2 and some of its proteins to induce innate memory in human monocytes in vitro. Our preliminary results show that the Spike protein subunits S1 and S2 and the entire heat-inactivated virus have no substantial effect. Conversely, monocytes pre-exposed to the nucleocapsid N protein react to subsequent viral or bacterial challenges with an increased production of anti-inflammatory IL-1Ra, a response profile suggesting a milder response to new infections.
摘要：
SARS-CoV-2与人类免疫系统的相互作用是感染的阳性或阴性结果的基础。单核细胞和巨噬细胞,它们是主要的先天免疫/炎症效应细胞，不会直接感染SARS-CoV-2，但是它们可以对病毒产生反应并引起强烈反应。这种第一次相互作用和反应是否会使先天反应性偏向于重新挑战，一种被称为先天记忆的现象，目前尚未开发，可能是COVID-19长期后遗症的一部分。这里,我们已经测试了SARS-CoV-2及其一些蛋白质在体外诱导人单核细胞先天记忆的能力。我们的初步结果表明，Spike蛋白亚基S1和S2以及整个热灭活病毒没有实质性影响。相反，预暴露于核衣壳N蛋白的单核细胞对随后的病毒或细菌挑战反应，产生增加的抗炎IL-1Ra，反应概况表明对新感染的反应较温和。
