Abstract：
The objective of this study was to determine whether a high-fat diet (HFD) fed to goats for a brief period during peri-conception would optimize reproductive and foetal responses. Thirty-four Anglo-Nubian crossbred adult goats were allocated into three groups: control (n = 11), fed with a total mixed ration (TMR) based on chopped elephant grass and concentrate; HFBM (n = 11), given TMR supplemented with soybean oil on a 0.5% dry matter basis for 11 days starting nine days before mating (BM); and HFAM (n = 12), fed with soybean oil included in the TMR for 15 days after mating (AM). The TMR diets differed in their fat content (7.5% vs. 2.9%). All goats had oestrus synchronized for 14 days BM by intravaginal administration of 60 mg MPA sponge for 12 days. Forty-eight hours BM, the sponge was removed and 0.075 mg PGF2α was applied intramuscularly. After 36 h, 1 ml GnRH was administered intramuscularly, and goats were mated after sponge removal. The fat groups showed lower feed intake (p < .001) and higher cholesterol levels (p < .001) when HFD was administered. Doppler and B-mode ultrasound evaluations revealed a greater (p < .05) number of small (<3 mm, 10 ± 0.6 vs. 8 ± 0.5) and large (≥3 mm, 6 ± 0.4 vs. 5.0 ± 0.2) follicles and intraovarian blood area (p < .05) in the HFBM group during sponge removal (57.6%) and mating (24.2%) than those of the no-fat group. During AM, the fat-fed groups exhibited higher glutathione peroxidase levels (p < .05) and a reduction (p < .001) in corpus luteum size (19%) and vascularized Doppler area (41%). No difference (p > .05) between groups was found in foetal traits, placentome and umbilical vascular development, except for the embryonic vesicle where HFAM twin pregnancy showed a smaller size than the control (26.1 ± 3.5 cm vs. 33.7 ± 2.7 cm; p < .01). Thus, HFD applied during peri-conception of goats has no impact on later foetal development but improved the follicular growth when given before the mating. Thus, the use of HFD in periconception has no impact on foetal development but increases follicular growth before breeding time.
摘要：
这项研究的目的是确定在受孕期间短暂喂养山羊的高脂肪饮食（HFD）是否会优化生殖和胎儿的反应。将34只盎格鲁-努比亚杂交成年山羊分为三组：对照组（n=11），饲喂基于切碎的象草和浓缩物的总混合日粮(TMR)；HFBM(n=11)，从交配前9天开始，给予TMR以0.5％干物质为基础补充大豆油11天（BM）；和HFAM（n=12），交配后（AM）用TMR中包含的大豆油喂养15天。TMR饮食的脂肪含量不同(7.5%与2.9%）。通过阴道内给予60mgMPA海绵12天，所有山羊的发情同步14天。四十八小时BM，取下海绵,肌内施用0.075mgPGF2α.36小时后，肌内注射1mlGnRH，和山羊在海绵去除后交配。当施用HFD时,脂肪组显示较低的采食量(p<.001)和较高的胆固醇水平(p<.001)。多普勒和B型超声评估显示更多（p<0.05）的小（<3mm，10±0.6vs.8±0.5)和大(≥3mm,6±0.4vs.5.0±0.2)个卵泡和卵巢内血面积(p<.05)HFBM组比无脂肪组(57.6%)和交配(24.2%)。在AM期间,脂肪喂养组的谷胱甘肽过氧化物酶水平较高(p<.05),黄体大小(19%)和血管化多普勒面积(41%)减少(p<.001).各组间胎儿性状无差异(p>0.05)，胎盘和脐血管发育，除了HFAM双胎妊娠的胚胎囊泡比对照组小（26.1±3.5cmvs.33.7±2.7厘米；p<.01）。因此，在山羊受孕期间应用HFD对以后的胎儿发育没有影响，但在交配前给予改善了卵泡生长。因此，在围知觉中使用HFD对胎儿发育没有影响，但在繁殖前增加了卵泡生长。
